| Reaction Details |
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 | Report a problem with these data |
| Target | Protein kinase YpkA |
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| Ligand | BDBM22043 |
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| Substrate/Competitor | myelin basic protein |
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| Meas. Tech. | Radiological Kinase Inhibition Assay |
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| pH | 7.5±n/a |
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| Temperature | 295.15±n/a K |
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| IC50 | 19840±4320 nM |
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| Citation |  Hu, X; Prehna, G; Stebbins, CE Targeting plague virulence factors: a combined machine learning method and multiple conformational virtual screening for the discovery of Yersinia protein kinase A inhibitors. J Med Chem50:3980-3 (2007) [PubMed] Article |
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| More Info.: | Get all data from this article, Solution Info, Assay Method |
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| Protein kinase YpkA |
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| Name: | Protein kinase YpkA |
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| Synonyms: | Protein kinase A | Targeted effector protein kinase | YPKA_YERPE | ypkA |
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| Type: | Serine/Threonine Protein Kinase |
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| Mol. Mass.: | 81763.80 |
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| Organism: | Yersinia pestis |
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| Description: | The YpkA gene was cloned from the Yersinia virulence plasmid (from J. Bliska, State University of New York at Stony Brook) as an N-terminal fusion with glutathione-S-transferase (GST). GST-YpkA was further purified by affinity chromatography using glutathione sepharose beads. |
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| Residue: | 732 |
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| Sequence: | MKSVKIMGTMPPSISLAKAHERISQHWQNPVGELNIGGKRYRIIDNQVLRLNPHSGFSLF
REGVGKIFSGKMFNFSIARNLTDTLHAAQKTTSQELRSDIPNALSNLFGAKPQTELPLGW
KGEPLSGAPDLEGMRVAETDKFAEGESHISIIETKDKQRLVAKIERSIAEGHLFAELEAY
KHIYKTAGKHPNLANVHGMAVVPYGNRKEEALLMDEVDGWRCSDTLRTLADSWKQGKINS
EAYWGTIKFIAHRLLDVTNHLAKAGVVHNDIKPGNVVFDRASGEPVVIDLGLHSRSGEQP
KGFTESFKAPELGVGNLGASEKSDVFLVVSTLLHCIEGFEKNPEIKPNQGLRFITSEPAH
VMDENGYPIHRPGIAGVETAYTRFITDILGVSADSRPDSNEARLHEFLSDGTIDEESAKQ
ILKDTLTGEMSPLSTDVRRITPKKLRELSDLLRTHLSSAATKQLDMGGVLSDLDTMLVAL
DKAEREGGVDKDQLKSFNSLILKTYRVIEDYVKGREGDTKNSSTEVSPYHRSNFMLSIVE
PSLQRIQKHLDQTHSFSDIGSLVRAHKHLETLLEVLVTLSQQGQPVSSETYGFLNRLTEA
KITLSQQLNTLQQQQESAKAQLSILINRSGSWADVARQSLQRFDSTRPVVKFGTEQYTAI
HRQMMAAHAAITLQEVSEFTDDMRNFTVDSIPLLIQLGRSSLMDEHLVEQREKLRELTTI
AERLNRLEREWM
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| BDBM22043 |
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| myelin basic protein |
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| Name: | myelin basic protein |
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| Synonyms: | MBP | MBP_HUMAN | Myelin A1 protein | Myelin membrane encephalitogenic protein | biotin-MBP protein | biotin-myelin basic protein |
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| Type: | Other Protein Type |
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| Mol. Mass.: | 33133.33 |
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| Organism: | Homo sapiens (Human) |
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| Description: | n/a |
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| Residue: | 304 |
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| Sequence: | MGNHAGKRELNAEKASTNSETNRGESEKKRNLGELSRTTSEDNEVFGEADANQNNGTSSQ
DTAVTDSKRTADPKNAWQDAHPADPGSRPHLIRLFSRDAPGREDNTFKDRPSESDELQTI
QEDSAATSESLDVMASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFG
GDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKG
RGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSP
MARR
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