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TargetDNA Ligase (MtuLigA)
LigandBDBM22984
Substrate/Competitor32P-labeled nicked duplex DNA substrate
Meas. Tech.In Vitro Ligase Activity Assay
pH8±n/a
Temperature298.15±n/a K
IC50 5000±300 nM
Citation Srivastava SKTripathi RPRamachandran R NAD+-dependent DNA Ligase (Rv3014c) from Mycobacterium tuberculosis. Crystal structure of the adenylation domain and identification of novel inhibitors. J Biol Chem 280:30273-81 (2005) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
DNA Ligase (MtuLigA)
Name:DNA Ligase (MtuLigA)
Synonyms:NAD-dependent DNA ligase | Polydeoxyribonucleotide synthase [NAD(+)] | Polydeoxyribonucleotide synthase [NAD+]
Type:Enzyme
Mol. Mass.:75276.13
Organism:Mycobacterium tuberculosis
Description:The C-terminally His-tagged full-length MtuLigA protein was cloned and expressed in E. coli.
Residue:691
Sequence:
MSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPE
LRTPDSPTQLVGGAGFATDFEPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLC
ELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTIADVPERLTPGDDYPVPEVLE
VRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGH
VEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVK
VDEVALQRRLGSTSRAPRWAIAYKYPPEEAQTKLLDIRVNVGRTGRITPFAFMTPVKVAG
STVGQATLHNASEIKRKGVLIGDTVVIRKAGDVIPEVLGPVVELRDGSEREFIMPTTCPE
CGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAGVALLQAKVIA
DEGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGPT
AARALATEFGSLDAIAAASTDQLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRM
VDERDESVPRTLAGLTIVVTGSLTGFSRDDAKEAIVARGGKAAGSVSKKTNYVVAGDSPG
SKYDKAVELGVPILDEDGFRRLLADGPASRT
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM22984
32P-labeled nicked duplex DNA substrate
Name:32P-labeled nicked duplex DNA substrate
Synonyms:n/a
Type:Duplex DNA
Mol. Mass.:3295.68
Organism:n/a
Description:n/a
Residue:40
Sequence:
5-ATG TCC AGT GAT CCA GCT AAG GTA CGA GTC TAT GTC CAG G-3