Reaction Details |
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Target | DNA ligase A |
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Ligand | BDBM22984 |
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Substrate/Competitor | 32P-labeled nicked duplex DNA substrate |
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Meas. Tech. | In Vitro Ligase Activity Assay |
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pH | 8±n/a |
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Temperature | 298.15±n/a K |
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IC50 | 5000±300 nM |
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Citation | Srivastava, SK; Tripathi, RP; Ramachandran, R NAD+-dependent DNA Ligase (Rv3014c) from Mycobacterium tuberculosis. Crystal structure of the adenylation domain and identification of novel inhibitors. J Biol Chem280:30273-81 (2005) [PubMed] Article |
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More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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DNA ligase A |
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Name: | DNA ligase A |
Synonyms: | DNA Ligase (MtuLigA) | DNLJ_MYCTU | NAD-dependent DNA ligase | Polydeoxyribonucleotide synthase [NAD(+)] | Polydeoxyribonucleotide synthase [NAD+] | lig | ligA |
Type: | Enzyme |
Mol. Mass.: | 75276.13 |
Organism: | Mycobacterium tuberculosis |
Description: | The C-terminally His-tagged full-length MtuLigA protein was cloned and expressed in E. coli. |
Residue: | 691 |
Sequence: | MSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPE
LRTPDSPTQLVGGAGFATDFEPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLC
ELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTIADVPERLTPGDDYPVPEVLE
VRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGH
VEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVK
VDEVALQRRLGSTSRAPRWAIAYKYPPEEAQTKLLDIRVNVGRTGRITPFAFMTPVKVAG
STVGQATLHNASEIKRKGVLIGDTVVIRKAGDVIPEVLGPVVELRDGSEREFIMPTTCPE
CGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAGVALLQAKVIA
DEGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGPT
AARALATEFGSLDAIAAASTDQLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRM
VDERDESVPRTLAGLTIVVTGSLTGFSRDDAKEAIVARGGKAAGSVSKKTNYVVAGDSPG
SKYDKAVELGVPILDEDGFRRLLADGPASRT
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BDBM22984 |
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32P-labeled nicked duplex DNA substrate |
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Name: | 32P-labeled nicked duplex DNA substrate |
Synonyms: | n/a |
Type: | Duplex DNA |
Mol. Mass.: | 3295.68 |
Organism: | n/a |
Description: | n/a |
Residue: | 40 |
Sequence: | 5-ATG TCC AGT GAT CCA GCT AAG GTA CGA GTC TAT GTC CAG G-3
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