Reaction Details |
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Target | DNA ligase |
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Ligand | BDBM22984 |
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Substrate/Competitor | 32P-labeled nicked duplex DNA substrate |
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Meas. Tech. | In Vitro Ligase Activity Assay |
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pH | 7.6±n/a |
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Temperature | 298.15±n/a K |
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IC50 | 3000±200 nM |
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Citation | Srivastava, SK; Tripathi, RP; Ramachandran, R NAD+-dependent DNA Ligase (Rv3014c) from Mycobacterium tuberculosis. Crystal structure of the adenylation domain and identification of novel inhibitors. J Biol Chem280:30273-81 (2005) [PubMed] Article |
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More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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DNA ligase |
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Name: | DNA ligase |
Synonyms: | 30 | ATP-dependent DNA ligase | Bacteriophage T4 DNA Ligase | DNLI_BPT4 | Polydeoxyribonucleotide synthase [ATP] |
Type: | Enzyme |
Mol. Mass.: | 55291.48 |
Organism: | Bacteriophage T4 |
Description: | n/a |
Residue: | 487 |
Sequence: | MILKILNEIASIGSTKQKQAILEKNKDNELLKRVYRLTYSRGLQYYIKKWPKPGIATQSF
GMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEVLRRVMMRDLECGASVS
IANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGARCFAEVRGDELDDVRLL
SRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHEQVKKEPEGLDFLFDAYPE
NSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQVWDYVPLVEIYSLPAFRLKY
DVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYIDQGLEGIILKNIDGLWENARS
KNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECGKIKVNAGSGLKDKAGVKSHEL
DRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFLPIAIRLREDKTKANTFEDVFGD
FHEVTGL
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BDBM22984 |
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32P-labeled nicked duplex DNA substrate |
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Name: | 32P-labeled nicked duplex DNA substrate |
Synonyms: | n/a |
Type: | Duplex DNA |
Mol. Mass.: | 3295.68 |
Organism: | n/a |
Description: | n/a |
Residue: | 40 |
Sequence: | 5-ATG TCC AGT GAT CCA GCT AAG GTA CGA GTC TAT GTC CAG G-3
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