| Reaction Details |
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 | Report a problem with these data |
| Target | DNA ligase |
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| Ligand | BDBM22985 |
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| Substrate/Competitor | 32P-labeled nicked duplex DNA substrate |
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| Meas. Tech. | In Vitro Ligase Activity Assay |
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| pH | 7.6±n/a |
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| Temperature | 298.15±n/a K |
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| IC50 | 1600000±15000 nM |
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| Citation |  Srivastava, SK; Dube, D; Kukshal, V; Jha, AK; Hajela, K; Ramachandran, R NAD+-dependent DNA ligase (Rv3014c) from Mycobacterium tuberculosis: novel structure-function relationship and identification of a specific inhibitor. Proteins69:97-111 (2007) [PubMed] Article |
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| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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| DNA ligase |
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| Name: | DNA ligase |
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| Synonyms: | 30 | ATP-dependent DNA ligase | Bacteriophage T4 DNA Ligase | DNLI_BPT4 | Polydeoxyribonucleotide synthase [ATP] |
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| Type: | Enzyme |
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| Mol. Mass.: | 55291.48 |
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| Organism: | Bacteriophage T4 |
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| Description: | n/a |
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| Residue: | 487 |
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| Sequence: | MILKILNEIASIGSTKQKQAILEKNKDNELLKRVYRLTYSRGLQYYIKKWPKPGIATQSF
GMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEVLRRVMMRDLECGASVS
IANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGARCFAEVRGDELDDVRLL
SRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHEQVKKEPEGLDFLFDAYPE
NSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQVWDYVPLVEIYSLPAFRLKY
DVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYIDQGLEGIILKNIDGLWENARS
KNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECGKIKVNAGSGLKDKAGVKSHEL
DRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFLPIAIRLREDKTKANTFEDVFGD
FHEVTGL
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| BDBM22985 |
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| 32P-labeled nicked duplex DNA substrate |
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| Name: | 32P-labeled nicked duplex DNA substrate |
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| Synonyms: | n/a |
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| Type: | Duplex DNA |
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| Mol. Mass.: | 3295.68 |
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| Organism: | n/a |
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| Description: | n/a |
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| Residue: | 40 |
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| Sequence: | 5-ATG TCC AGT GAT CCA GCT AAG GTA CGA GTC TAT GTC CAG G-3
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