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TargetBotulinum neurotoxin type A2 [1-425]
LigandBDBM23300
Substrate/CompetitorSNAP-25 substrate peptide
Meas. Tech.BoNT/A LC Metalloprotease Activity Assay
pH7.3±n/a
Temperature295.15±n/a K
Comments12% inhibition uM.
Citation Burnett, JC; Opsenica, D; Sriraghavan, K; Panchal, RG; Ruthel, G; Hermone, AR; Nguyen, TL; Kenny, TA; Lane, DJ; McGrath, CF; Schmidt, JJ; Vennerstrom, JL; Gussio, R; Solaja, BA; Bavari, S A refined pharmacophore identifies potent 4-amino-7-chloroquinoline-based inhibitors of the botulinum neurotoxin serotype A metalloprotease. J Med Chem50:2127-36 (2007) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Botulinum neurotoxin type A2 [1-425]
Name:Botulinum neurotoxin type A2 [1-425]
Synonyms:BXA2_CLOBJ | BoNT/A LC | Botulinum Neurotoxin Type A | Botulinum neurotoxin A light chain | atx | bna | bonT | bont/a2 | botA
Type:Metalloprotease
Mol. Mass.:48687.43
Organism:Clostridium botulinum
Description:Recombinant C. botulinum LC/A (1-425) was expressed in E. coli.
Residue:425
Sequence:
MPFVNKQFNYKDPVNGVDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLN
PPPEAKQVPVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGG
STIDTELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHDVLNLTRNGY
GSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAEHRLYGIAINPN
RVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDVASTLNKA
KSIIGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVNFFKV
INRKTYLNFDKAVFRINIVPDENYTIKDGFNLKGANLSTNFNGQNTEINSRNFTRLKNFT
GLFEF
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  Blast E-value cutoff:
BDBM23300
SNAP-25 substrate peptide
Name:SNAP-25 substrate peptide
Synonyms:n/a
Type:Peptide
Mol. Mass.:3661.13
Organism:n/a
Description:The substrate peptide consisted of residues 187-203 of synaptosomes associated protein (SNAP-25).
Residue:32
Sequence:
(N-acetyl)-SNKTRIDEANQRATKML-(carboxamide)