| Reaction Details |
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 | Report a problem with these data |
| Target | Polyunsaturated fatty acid 5-lipoxygenase |
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| Ligand | BDBM24566 |
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| Substrate/Competitor | BDBM22319 |
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| Meas. Tech. | Determination of 5-LO Product Formation in Intact Cells (EC50/IC50) |
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| pH | 7.4±n/a |
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| Temperature | 310.15±n/a K |
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| Comments | No inhibition of 5-LO product formation in Intact cells @ 10 uM. |
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| Citation |  Werz, O; Greiner, C; Koeberle, A; Hoernig, C; George, S; Popescu, L; Syha, I; Schubert-Zsilavecz, M; Steinhilber, D Novel and potent inhibitors of 5-lipoxygenase product synthesis based on the structure of pirinixic acid. J Med Chem51:5449-53 (2008) [PubMed] Article |
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| More Info.: | Get all data from this article, Solution Info, Assay Method |
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| Polyunsaturated fatty acid 5-lipoxygenase |
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| Name: | Polyunsaturated fatty acid 5-lipoxygenase |
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| Synonyms: | 5-LO | 5-Lipo-oxygenase (5-LOX) | 5-Lipoxygenase (5-LO) | 5-Lipoxygenase (LOX) | 5-Lipoygenase | 5-lipoxygenase/FLAP | ALOX5 | Arachidonate 5-lipoxygenase | LOG5 | LOX5_HUMAN |
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| Type: | Enzyme |
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| Mol. Mass.: | 77972.74 |
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| Organism: | Homo sapiens (Human) |
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| Description: | Recombinant protein was purified from E. coli lysate. After ammonium sulfate precipitation and subsequent steps, the supernatant (S100) was used for 5-LO activity assay.
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| Residue: | 674 |
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| Sequence: | MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDE
ELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLA
RDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVL
NYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNG
CNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDP
CTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDF
HVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECG
LFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWE
AIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYL
TVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCW
HLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPY
YYLSPDRIPNSVAI
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| BDBM24566 |
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| BDBM22319 |
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| Name | BDBM24566 |
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| Synonyms: | 2-({4-chloro-6-[(2,3-dimethylphenyl)amino]pyrimidin-2-yl}sulfanyl)acetic acid | CHEMBL295416 | JMC515449 Compound 1 | Pirinixic acid | Wyeth 14,643 |
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| Type | Small organic molecule |
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| Emp. Form. | C14H14ClN3O2S |
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| Mol. Mass. | 323.798 |
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| SMILES | Cc1cccc(Nc2cc(Cl)nc(SCC(O)=O)n2)c1C |
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| Structure | 
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