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TargetRho-associated protein kinase 2 [1-543,T431N]
LigandBDBM25472
Substrate/CompetitorSTK substrate 2-biotin
Meas. Tech.ROCK-II Inhibition Assay
pH7±n/a
Temperature295.15±n/a K
IC50 1.5±0.7 nM
Citation Feng, Y; Yin, Y; Weiser, A; Griffin, E; Cameron, MD; Lin, L; Ruiz, C; Schürer, SC; Inoue, T; Rao, PV; Schröter, T; Lograsso, P Discovery of substituted 4-(pyrazol-4-yl)-phenylbenzodioxane-2-carboxamides as potent and highly selective Rho kinase (ROCK-II) inhibitors. J Med Chem51:6642-5 (2008) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Rho-associated protein kinase 2 [1-543,T431N]
Name:Rho-associated protein kinase 2 [1-543,T431N]
Synonyms:KIAA0619 | ROCK2 | ROCK2_HUMAN | Rho-associated, coiled-coil-containing protein kinase 2 | Rho-kinase (ROCK II) | p164 ROCK-2
Type:Serine/threonine-protein kinase
Mol. Mass.:62266.25
Organism:Homo sapiens (Human)
Description:The truncated human Rho-kinase II (1-543) expressed in Sf-21 cell.
Residue:543
Sequence:
MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFP
ALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAM
KLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNL
MSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMD
ETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLV
GTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWH
WDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENL
LLSDSPSCRENDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKT
AKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQL
EDL
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM25472
STK substrate 2-biotin
Name:STK substrate 2-biotin
Synonyms:n/a
Type:biotinylated peptide substrate
Mol. Mass.:358.43
Organism:n/a
Description:HTRF KinEASE-STK S2 Assay Kit (Cisbio, Bedford, MA)
Residue:3
Sequence:
n/a