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TargetGag-Pol polyprotein [588-1027,V694A]/[588-1147,V694A]
LigandBDBM1434
Substrate/Competitorpoly(rA)/oligo(dT)
Meas. Tech.Reverse Transcriptase Assay
Ki 10000±n/a nM
Citation Butini, S; Brindisi, M; Cosconati, S; Marinelli, L; Borrelli, G; Coccone, SS; Ramunno, A; Campiani, G; Novellino, E; Zanoli, S; Samuele, A; Giorgi, G; Bergamini, A; Mattia, MD; Lalli, S; Galletti, B; Gemma, S; Maga, G Specific Targeting of Highly Conserved Residues in the HIV-1 Reverse Transcriptase Primer Grip Region. 2. Stereoselective Interaction to Overcome the Effects of Drug Resistant Mutations. J Med Chem52:1224-8 (2009) [PubMed]  Article
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Gag-Pol polyprotein [588-1027,V694A]/[588-1147,V694A]
Name:Gag-Pol polyprotein [588-1027,V694A]/[588-1147,V694A]
Synonyms:HIV-1 Reverse Transcriptase Mutant (V106A)
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [588-1147,V694A]
Synonyms:HIV-1 Reverse Transcriptase Mutant (V106A) Chain A | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:64458.36
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1147,V694A]
Residue:560
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSATVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGIRKVL
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Component 2
Name:Gag-Pol polyprotein [588-1027,V694A]
Synonyms:HIV-1 Reverse Transcriptase Mutant (V106A) Chain B | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:51309.07
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1027,V694A]
Residue:440
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSATVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETF
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BDBM1434
poly(rA)/oligo(dT)
Name:poly(rA)/oligo(dT)
Synonyms:n/a
Type:RNA/DNA duplex
Mol. Mass.:358.43
Organism:n/a
Description:[3H]-dTTP is co-substrate.
Residue:3
Sequence:
n/a