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TargetGag-Pol polyprotein [588-1027,K691N,Y769C]/[588-1147,K691N,Y769C]
LigandBDBM27614
Substrate/CompetitorRNA:Biotin-DNA template:primer
Meas. Tech.HIV-1 Reverse Transcriptase Assay
pH8±n/a
Temperature303.15±n/a K
IC50 3±n/a nM
EC50 4±n/a nM
Citation Sweeney, ZK; Harris, SF; Arora, SF; Javanbakht, H; Li, Y; Fretland, J; Davidson, JP; Billedeau, JR; Gleason, SK; Hirschfeld, D; Kennedy-Smith, JJ; Mirzadegan, T; Roetz, R; Smith, M; Sperry, S; Suh, JM; Wu, J; Tsing, S; Villaseñor, AG; Paul, A; Su, G; Heilek, G; Hang, JQ; Zhou, AS; Jernelius, JA; Zhang, FJ; Klumpp, K Design of annulated pyrazoles as inhibitors of HIV-1 reverse transcriptase. J Med Chem51:7449-58 (2008) [PubMed]  Article
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Gag-Pol polyprotein [588-1027,K691N,Y769C]/[588-1147,K691N,Y769C]
Name:Gag-Pol polyprotein [588-1027,K691N,Y769C]/[588-1147,K691N,Y769C]
Synonyms:HIV-1 Reverse Transcriptase Mutant (103N/181C)
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [588-1147,K691N,Y769C]
Synonyms:HIV-1 Reverse Transcriptase Mutant (103N/181C) Chain A | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:64411.30
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1147,K691N,Y769C]
Residue:560
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKNKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
CQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGIRKVL
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Component 2
Name:Gag-Pol polyprotein [588-1027,K691N,Y769C]
Synonyms:HIV-1 Reverse Transcriptase Mutant (103N/181C) Chain B | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:51262.02
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1027,K691N,Y769C]
Residue:440
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKNKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
CQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETF
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BDBM27614
RNA:Biotin-DNA template:primer
Name:RNA:Biotin-DNA template:primer
Synonyms:n/a
Type:RNA/DNA duplex
Mol. Mass.:2957.49
Organism:n/a
Description:The incorporation of [3H]dATP onto a RNA:(biotin)DNA template:primer.
Residue:38
Sequence:
5-GGG UCU CUC UGG UUA GAC CAC UCU AGC AGU GGC GCC CGA ACA GG
G AC-3