| Reaction Details |
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 | Report a problem with these data |
| Target | Gag-Pol polyprotein [1148-1435] |
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| Ligand | BDBM27749 |
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| Substrate/Competitor | DIG-tagged Target DNA |
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| Meas. Tech. | DNA Strand-Transfer Activity Assay |
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| pH | 7.3±n/a |
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| Temperature | 295.15±n/a K |
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| IC50 | 14±n/a nM |
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| Citation |  Metobo, S; Mish, M; Jin, H; Jabri, S; Lansdown, R; Chen, X; Tsiang, M; Wright, M; Kim, CU Tricyclic HIV integrase inhibitors: VI. SAR studies of 'benzyl flipped' C3-substituted pyrroloquinolines. Bioorg Med Chem Lett19:1187-90 (2009) [PubMed] Article |
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| More Info.: | Get all data from this article, Solution Info, Assay Method |
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| Gag-Pol polyprotein [1148-1435] |
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| Name: | Gag-Pol polyprotein [1148-1435] |
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| Synonyms: | HIV-1 Integrase | POL_HV1H2 | gag-pol |
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| Type: | Enzyme |
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| Mol. Mass.: | 32171.42 |
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| Organism: | Human immunodeficiency virus type 1 |
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| Description: | The sequence encoding the 288-amino-acid wild-type full-length integrase was cloned with an N-terminal 6His tag, and expressed in E. coli. |
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| Residue: | 288 |
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| Sequence: | FLDGIDKAQDEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPGI
WQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTIHTDNGSN
FTGATVRAACWWAGIKQEFGIPYNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAV
FIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRNPLWKGPAK
LLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDED
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| BDBM27749 |
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| DIG-tagged Target DNA |
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| Name: | DIG-tagged Target DNA |
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| Synonyms: | digoxin-tagged DNA |
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| Type: | Duplex DNA |
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| Mol. Mass.: | 1978.21 |
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| Organism: | n/a |
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| Description: | The biotinylated donor DNA was bound to streptavidin-coated plates. |
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| Residue: | 23 |
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| Sequence: | 5-TGACCAAGGGCTAATTCACT-3-DIG
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