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TargetDimer of Gag-Pol polyprotein [489-587]
LigandBDBM544
Substrate/CompetitorPeptide Substrate
Meas. Tech.Protease Inhibition Assay
pH5±n/a
Temperature295.15±n/a K
Ki 17±n/a nM
Citation Thaisrivongs, SSkulnick, HITurner, SRStrohbach, JWTommasi, RAJohnson, PDAristoff, PAJudge, TMGammill, RBMorris, JKRomines, KRChrusciel, RAHinshaw, RRChong, KTTarpley, WGPoppe, SMSlade, DELynn, JCHorng, MMTomich, PKSeest, EPDolak, LAHowe, WJHoward, GMWatenpaugh, KD Structure-based design of HIV protease inhibitors: sulfonamide-containing 5,6-dihydro-4-hydroxy-2-pyrones as non-peptidic inhibitors. J Med Chem39:4349-53 (1996) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Dimer of Gag-Pol polyprotein [489-587]
Name:Dimer of Gag-Pol polyprotein [489-587]
Synonyms:HIV-1 Protease
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [489-587]
Synonyms:HIV protease | HIV-1 Protease (NY5-type sequence) | HIV-1 Protease chain A | HIV-1 Protease chain B | POL_HV1N5 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10822.21
Organism:Human immunodeficiency virus type 1
Description:P12497[489-587]
Residue:99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
Component 2
Name:Gag-Pol polyprotein [489-587]
Synonyms:HIV protease | HIV-1 Protease (NY5-type sequence) | HIV-1 Protease chain A | HIV-1 Protease chain B | POL_HV1N5 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10822.21
Organism:Human immunodeficiency virus type 1
Description:P12497[489-587]
Residue:99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM544
Peptide Substrate
Name:Peptide Substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:1447.64
Organism:n/a
Description:The peptide was derivatized with biotin and fluorescein isothiocyanate at the amino and carboxy termini.
Residue:12
Sequence:
RVSQNYPIVQNK