| Reaction Details |
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 | Report a problem with these data |
| Target | Histone deacetylase |
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| Ligand | BDBM29603 |
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| Substrate/Competitor | pfHDAC-1 Substrate |
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| Meas. Tech. | pfHDAC-1 Enzyme Assay |
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| pH | 7.5±n/a |
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| Temperature | 296.15±n/a K |
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| IC50 | 36±6 nM |
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| Km | 30000±2000 nM |
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| kcat | 0.19±0.01 1/sec |
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| Citation |  Patel, V; Mazitschek, R; Coleman, B; Nguyen, C; Urgaonkar, S; Cortese, J; Barker, RH; Greenberg, E; Tang, W; Bradner, JE; Schreiber, SL; Duraisingh, MT; Wirth, DF; Clardy, J Identification and characterization of small molecule inhibitors of a class I histone deacetylase from Plasmodium falciparum. J Med Chem52:2185-7 (2009) [PubMed] Article |
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| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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| |
| Histone deacetylase |
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| Name: | Histone deacetylase |
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| Synonyms: | Histone Deacetylase (pfHDAC-1) |
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| Type: | Enzyme |
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| Mol. Mass.: | 51371.91 |
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| Organism: | Plasmodium falciparum (isolate 3D7) |
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| Description: | Assays were performed using recombinant pfHDAC-1 expressed and purified from S2 insect cells. |
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| Residue: | 449 |
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| Sequence: | MSNRKKVAYFHDPDIGSYYYGAGHPMKPQRIRMTHSLIVSYNLYKYMEVYRPHKSDVNEL
TLFHDYEYIDFLSSISLENYREFTYQLKRFNVGEATDCPVFDGLFQFQQSCAGASIDGAS
KLNHHCADICVNWSGGLHHAKMSEASGFCYINDIVLGILELLKYHARVMYIDIDVHHGDG
VEEAFYVTHRVMTVSFHKFGDYFPGTGDITDVGVNHGKYYSVNVPLNDGMTDDAFVDLFK
VVIDKCVQTYRPGAIIIQCGADSLTGDRLGRFNLTIKGHARCVEHVRSYNIPLLVLGGGG
YTIRNVSRCWAYETGVVLNKHHEMPDQISLNDYYDYYAPDFQLHLQPSNIPNYNSPEHLS
RIKMKIAENLRHIEHAPGVQFSYVPPDFFNSDIDDESDKNQYELKDDSGGGRAPGTRAKE
HSTTHHLRRKNYDDDFFDLSDRDQSIVPY
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| BDBM29603 |
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| pfHDAC-1 Substrate |
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| Name: | pfHDAC-1 Substrate |
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| Synonyms: | n/a |
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| Type: | fluorogenic, acetylated peptide |
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| Mol. Mass.: | 1609.93 |
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| Organism: | n/a |
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| Description: | n/a |
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| Residue: | 15 |
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| Sequence: | |
|---|
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