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TargetInterstitial collagenase
LigandBDBM30347
Substrate/CompetitorNFF-2 Substrate
Meas. Tech.Determination of MMP-13 Activity
Temperature298.15±n/a K
IC50>10000±n/a nM
Commentsextracted
Citation Monovich LGTommasi RAFujimoto RABlancuzzi VClark KCornell WDDoti RDoughty JFang JFarley DFitt JGanu VGoldberg RGoldstein RLavoie SKulathila RMacchia WParker DTMelton RO'Byrne EPastor GPellas TQuadros EReel NRoland DMSakane YSingh HSkiles JSomers JToscano KWigg AZhou SZhu LShieh WCXue SMcQuire LW Discovery of potent, selective, and orally active carboxylic acid based inhibitors of matrix metalloproteinase-13. J Med Chem 52:3523-38 (2009) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Interstitial collagenase
Name:Interstitial collagenase
Synonyms:Fibroblast collagenase | MMP1 | Matrix metalloproteinase-1 | Matrix metalloproteinase-1 (MMP-1)
Type:Enzyme
Mol. Mass.:54010.26
Organism:Homo sapiens (Human)
Description:
Residue:469
Sequence:
MHSFPPLLLLLFWGVVSHSFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPV
VEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIEN
YTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGN
LAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSY
TFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDR
FYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGY
PKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFP
GIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN
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  Blast E-value cutoff:
BDBM30347
NFF-2 Substrate
Name:NFF-2 Substrate
Synonyms:n/a
Type:Fluorogenic Substrate
Mol. Mass.:2346.23
Organism:n/a
Description:n/a
Residue:20
Sequence:
Mca-RPKPYA-Nva-WM-K(Dnp)-NH2