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TargetE3 ubiquitin-protein ligase Mdm2
LigandBDBM31223
Substrate/Competitor6-FAM labeled octapeptide
Meas. Tech.Fluorescence Polarization (FP) Binding Assay
pH7.4±n/a
Temperature296.15±n/a K
Ki 10000±n/a nM
Citation Zhang, R; Mayhood, T; Lipari, P; Wang, Y; Durkin, J; Syto, R; Gesell, J; McNemar, C; Windsor, W Fluorescence polarization assay and inhibitor design for MDM2/p53 interaction. Anal Biochem331:138-46 (2004) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
E3 ubiquitin-protein ligase Mdm2
Name:E3 ubiquitin-protein ligase Mdm2
Synonyms:Double minute 2 protein | Double minute 2 protein (HDM2) | E3 ubiquitin-protein ligase Mdm2 (p53-binding protein Mdm2) | Hdm2 | Human Double Minute 2 (HDM2) | MDM2 | MDM2-MDMX | MDM2_HUMAN | p53-Binding Protein MDM2 | p53-binding protein
Type:Oncoprotein
Mol. Mass.:55196.54
Organism:Homo sapiens (Human)
Description:Q00987
Residue:491
Sequence:
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQY
IMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGT
SVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQ
RKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDS
VSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLA
DYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVP
DCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQ
DKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQ
PIQMIVLTYFP
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM31223
6-FAM labeled octapeptide
Name:6-FAM labeled octapeptide
Synonyms:n/a
Type:fluorescent peptide
Mol. Mass.:2832.21
Organism:n/a
Description:Fluorophore 6-carboxyfluorescein was covalently attached to the side chain amino group of the Dpr residue in the peptide.
Residue:26
Sequence:
Ac-Phe-Arg-Dpr-Ac6c-(6-Br)Trp-Glu-Glu-Leu-NH2