Ki Summary BindingDB logo
myBDB logout
Reaction Details
Report a problem with these data
TargetCollagenase 3
LigandBDBM32414
Substrate/Competitor5-FAM/QXL 520 Peptide Substrate
Meas. Tech.Fluorescence Resonance Energy Transfer (FRET) Assay
pH7.5±n/a
Temperature310.15±n/a K
IC50 6±n/a nM
Citation Heim-Riether, A; Taylor, SJ; Liang, S; Gao, DA; Xiong, Z; Michael August, E; Collins, BK; Farmer, BT; Haverty, K; Hill-Drzewi, M; Junker, HD; Mariana Margarit, S; Moss, N; Neumann, T; Proudfoot, JR; Keenan, LS; Sekul, R; Zhang, Q; Li, J; Farrow, NA Improving potency and selectivity of a new class of non-Zn-chelating MMP-13 inhibitors. Bioorg Med Chem Lett19:5321-4 (2009) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Collagenase 3
Name:Collagenase 3
Synonyms:MMP-13 | MMP13 | MMP13_HUMAN | Matrix metalloproteinase-13 | Matrix metalloproteinase-13 (MMP-13) | Matrix metalloproteinase-13 (MMP13)
Type:Enzyme
Mol. Mass.:53808.06
Organism:Homo sapiens (Human)
Description:P45452
Residue:471
Sequence:
MHPGVLAAFLFLSWTHCRALPLPSGGDEDDLSEEDLQFAERYLRSYYHPTNLAGILKENA
ASSMTERLREMQSFFGLEVTGKLDDNTLDVMKKPRCGVPDVGEYNVFPRTLKWSKMNLTY
RIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDG
PSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALM
FPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGET
MIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGY
DILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLI
EEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM32414
5-FAM/QXL 520 Peptide Substrate
Name:5-FAM/QXL 520 Peptide Substrate
Synonyms:n/a
Type:FRET Peptide
Mol. Mass.:1848.68
Organism:n/a
Description:EnzoLyte520 Generic MMP Assay Kit (AnaSpec Inc.).
Residue:15
Sequence:
QXL520-PLAYWAR-K(5-FAM)-NH2