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TargetDimer of Gag-Pol polyprotein [501-599,D531N,M537I]
LigandBDBM520
Substrate/CompetitorChromogenic peptide substrate
Meas. Tech.Protease Inhibition Assay
pH4.7±n/a
Temperature310.15±n/a K
Ki 4.5±0.3 nM
Km22000±2000 nM
kcat4.9±0.3 1/sec
Citation Clemente, JCHemrajani, RBlum, LEGoodenow, MMDunn, BM Secondary mutations M36I and A71V in the human immunodeficiency virus type 1 protease can provide an advantage for the emergence of the primary mutation D30N. Biochemistry42:15029-35 (2003) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Dimer of Gag-Pol polyprotein [501-599,D531N,M537I]
Name:Dimer of Gag-Pol polyprotein [501-599,D531N,M537I]
Synonyms:HIV-1 Protease Mutant (D30N/M36I)
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [501-599,D531N,M537I]
Synonyms:HIV-1 Protease Mutant (D30N/M36I) chain A | HIV-1 Protease Mutant (D30N/M36I) chain B | LAI (D30N/M36I) | POL_HV1BR | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10777.17
Organism:Human immunodeficiency virus type 1
Description:P03367[501-599,D531N,M537I]
Residue:99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADNTVLEEISLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
Component 2
Name:Gag-Pol polyprotein [501-599,D531N,M537I]
Synonyms:HIV-1 Protease Mutant (D30N/M36I) chain A | HIV-1 Protease Mutant (D30N/M36I) chain B | LAI (D30N/M36I) | POL_HV1BR | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10777.17
Organism:Human immunodeficiency virus type 1
Description:P03367[501-599,D531N,M537I]
Residue:99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADNTVLEEISLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM520
Chromogenic peptide substrate
Name:Chromogenic peptide substrate
Synonyms:Chromogenic peptide substrate
Type:Peptide
Mol. Mass.:1420.11
Organism:n/a
Description:n/a
Residue:13
Sequence:
K-A-R-V-L#Nph-E-A-nL-G