Reaction Details |
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Target | Dimer of Gag-Pol polyprotein [501-599,D531N,M537I] |
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Ligand | BDBM520 |
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Substrate/Competitor | Chromogenic peptide substrate |
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Meas. Tech. | Protease Inhibition Assay |
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pH | 4.7±n/a |
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Temperature | 310.15±n/a K |
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Ki | 4.5±0.3 nM |
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Km | 22000±2000 nM |
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kcat | 4.9±0.3 1/sec |
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Citation | Clemente, JC; Hemrajani, R; Blum, LE; Goodenow, MM; Dunn, BM Secondary mutations M36I and A71V in the human immunodeficiency virus type 1 protease can provide an advantage for the emergence of the primary mutation D30N. Biochemistry42:15029-35 (2003) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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Dimer of Gag-Pol polyprotein [501-599,D531N,M537I] |
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Name: | Dimer of Gag-Pol polyprotein [501-599,D531N,M537I] |
Synonyms: | HIV-1 Protease Mutant (D30N/M36I) |
Type: | Protein Complex |
Mol. Mass.: | n/a |
Description: | n/a |
Components: | This complex has 2 components. |
Component 1 |
Name: | Gag-Pol polyprotein [501-599,D531N,M537I] |
Synonyms: | HIV-1 Protease Mutant (D30N/M36I) chain A | HIV-1 Protease Mutant (D30N/M36I) chain B | LAI (D30N/M36I) | POL_HV1BR | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10777.17 |
Organism: | Human immunodeficiency virus type 1 |
Description: | P03367[501-599,D531N,M537I] |
Residue: | 99 |
Sequence: | PQITLWQRPLVTIKIGGQLKEALLDTGADNTVLEEISLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
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Component 2 |
Name: | Gag-Pol polyprotein [501-599,D531N,M537I] |
Synonyms: | HIV-1 Protease Mutant (D30N/M36I) chain A | HIV-1 Protease Mutant (D30N/M36I) chain B | LAI (D30N/M36I) | POL_HV1BR | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10777.17 |
Organism: | Human immunodeficiency virus type 1 |
Description: | P03367[501-599,D531N,M537I] |
Residue: | 99 |
Sequence: | PQITLWQRPLVTIKIGGQLKEALLDTGADNTVLEEISLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
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BDBM520 |
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Chromogenic peptide substrate |
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Name: | Chromogenic peptide substrate |
Synonyms: | Chromogenic peptide substrate |
Type: | Peptide |
Mol. Mass.: | 1420.11 |
Organism: | n/a |
Description: | n/a |
Residue: | 13 |
Sequence: | |