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TargetRibonuclease H1
LigandBDBM33417
Substrate/CompetitorRNA/DNA hybrid
Meas. Tech.Fluorescence-Based RNase H Assay
pH8±n/a
Temperature293.15±n/a K
IC50>100000±n/a nM
Citation Kirschberg TABalakrishnan MSquires NHBarnes TBrendza KMChen XEisenberg EJJin WKutty NLeavitt SLiclican ALiu QLiu XMak JPerry JKWang MWatkins WJLansdon EB RNase H active site inhibitors of human immunodeficiency virus type 1 reverse transcriptase: design, biochemical activity, and structural information. J Med Chem 52:5781-4 (2009) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Ribonuclease H1
Name:Ribonuclease H1
Synonyms:RNase H1 | Ribonuclease H type II
Type:Enzyme
Mol. Mass.:32076.45
Organism:Homo sapiens (Human)
Description:n/a
Residue:286
Sequence:
MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKK
FATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKP
SVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQ
TNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVIN
KEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED
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  Blast E-value cutoff:
BDBM33417
RNA/DNA hybrid
Name:RNA/DNA hybrid
Synonyms:n/a
Type:fluorescein-labeled RNA annealed to DNA
Mol. Mass.:2134.40
Organism:n/a
Description:It is a 18-nucleotide 3-fluorescein-labeled RNA annealed to a complementary 18-nucleotide 5-Dabcyl-modified DNA.
Residue:23
Sequence:
5-GAU CUG AGC CUG GGA GCU-fluorescein-3