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TargetGag-Pol polyprotein [600-1028,C879S]/[600-1151,C879S]
LigandBDBM33954
Substrate/Competitorpoly(rA)-oligo(dT)(12-18)
Meas. Tech.Assay of RT RNA-Dependent DNA Polymerase (RDDP) Activity
pH7.8±n/a
Temperature310.15±n/a K
IC50>50000±n/a nM
Citation Himmel, DM; Sarafianos, SG; Dharmasena, S; Hossain, MM; McCoy-Simandle, K; Ilina, T; Clark, AD; Knight, JL; Julias, JG; Clark, PK; Krogh-Jespersen, K; Levy, RM; Hughes, SH; Parniak, MA; Arnold, E HIV-1 reverse transcriptase structure with RNase H inhibitor dihydroxy benzoyl naphthyl hydrazone bound at a novel site. ACS Chem Biol1:702-12 (2006) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Gag-Pol polyprotein [600-1028,C879S]/[600-1151,C879S]
Name:Gag-Pol polyprotein [600-1028,C879S]/[600-1151,C879S]
Synonyms:HIV-1 Reverse Transcriptase RNase H
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [600-1151,C879S]
Synonyms:HIV-1 Reverse Transcriptase RNase H Chain A | POL_HV1B1 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:63584.38
Organism:Human immunodeficiency virus type 1
Description:P03366[600-1151,C879S]
Residue:552
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNKGRQKVVPLTNTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLV
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Component 2
Name:Gag-Pol polyprotein [600-1028,C879S]
Synonyms:HIV-1 Reverse Transcriptase RNase H Chain B | POL_HV1B1 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:50093.74
Organism:Human immunodeficiency virus type 1
Description:P03366[600-1028,C879S]
Residue:429
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQL
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BDBM33954
poly(rA)-oligo(dT)(12-18)
Name:poly(rA)-oligo(dT)(12-18)
Synonyms:homopolymeric template-primer
Type:RNA/DNA Duplex
Mol. Mass.:358.43
Organism:n/a
Description:[3H]-TTP as co-substrate.
Residue:3
Sequence:
n/a