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TargetGag-Pol polyprotein [613-1167,R896S,E946D,S1117V]/[618-1043,R896S,E946D]
LigandBDBM33045
Substrate/CompetitorRNA/DNA hybrid
Meas. Tech.FRET-Based RNase H Assay
pH8±n/a
Temperature293.15±n/a K
IC50 2500±n/a nM
Citation Wendeler, M; Lee, HF; Bermingham, A; Miller, JT; Chertov, O; Bona, MK; Baichoo, NS; Ehteshami, M; Beutler, J; O'Keefe, BR; Götte, M; Kvaratskhelia, M; Le Grice, S Vinylogous ureas as a novel class of inhibitors of reverse transcriptase-associated ribonuclease H activity. ACS Chem Biol3:635-44 (2008) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Gag-Pol polyprotein [613-1167,R896S,E946D,S1117V]/[618-1043,R896S,E946D]
Name:Gag-Pol polyprotein [613-1167,R896S,E946D,S1117V]/[618-1043,R896S,E946D]
Synonyms:HIV-2 Reverse Transcriptase | HIV-2 Reverse Transcriptase RNase H
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [613-1167,R896S,E946D,S1117V]
Synonyms:HIV-2 Reverse Transcriptase Chain A | HIV-2 Reverse Transcriptase RNase H Chain A | POL_HV2RO | gag-pol
Type:Enzyme Subunit
Mol. Mass.:64085.94
Organism:Human immunodeficiency virus type 2
Description:P04584[613-1167,R896S,E946D,S1117V]
Residue:555
Sequence:
PVAKVEPIKIMLKPGKDGPKLRQWPLTKEKIEALKEICEKMEKEGQLEEAPPTNPYNTPT
FAIKKKDKNKWRMLIDFRELNKVTQDFTEIQLGIPHPAGLAKKRRITVLDVGDAYFSIPL
HEDFRPYTAFTLPSVNNAEPGKRYIYKVLPQGWKGSPAIFQHTMRQVLEPFRKANKDVII
IQYMDDILIASDRTDLEHDRVVLQLKELLNGLGFSTPDEKFQKDPPYHWMGYELWPTKWK
LQKIQLPQKEIWTVNDIQKLVGVLNWAAQLYPGIKTKHLCRLISGKMTLTEEVQWTELAE
AELEENRIILSQEQEGHYYQEEKELEATVQKDQDNQWTYKIHQEEKILKVGKYAKVKNTH
TNGIRLLAQVVQKIGKEALVIWGRIPKFHLPVEREIWEQWWDNYWQVTWIPDWDFVSTPP
LVRLAFNLVGDPIPGAETFYTDGSCNRQSKEGKAGYVTDRGKDKVKKLEQTTNQQAELEA
FAMALTDSGPKVNIIVDSQYVMGIVASQPTESESKIVNQIIEEMIKKEAIYVAWVPAHKG
IGGNQEVDHLVSQGI
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Component 2
Name:Gag-Pol polyprotein [618-1043,R896S,E946D]
Synonyms:HIV-2 Reverse Transcriptase Chain B | HIV-2 Reverse Transcriptase RNase H Chain B | POL_HV2RO | gag-pol
Type:Enzyme Subunit
Mol. Mass.:50116.27
Organism:Human immunodeficiency virus type 2
Description:P04584[618-1043,R896S,E946D]
Residue:426
Sequence:
EPIKIMLKPGKDGPKLRQWPLTKEKIEALKEICEKMEKEGQLEEAPPTNPYNTPTFAIKK
KDKNKWRMLIDFRELNKVTQDFTEIQLGIPHPAGLAKKRRITVLDVGDAYFSIPLHEDFR
PYTAFTLPSVNNAEPGKRYIYKVLPQGWKGSPAIFQHTMRQVLEPFRKANKDVIIIQYMD
DILIASDRTDLEHDRVVLQLKELLNGLGFSTPDEKFQKDPPYHWMGYELWPTKWKLQKIQ
LPQKEIWTVNDIQKLVGVLNWAAQLYPGIKTKHLCRLISGKMTLTEEVQWTELAEAELEE
NRIILSQEQEGHYYQEEKELEATVQKDQDNQWTYKIHQEEKILKVGKYAKVKNTHTNGIR
LLAQVVQKIGKEALVIWGRIPKFHLPVEREIWEQWWDNYWQVTWIPDWDFVSTPPLVRLA
FNLVGD
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BDBM33045
RNA/DNA hybrid
Name:RNA/DNA hybrid
Synonyms:n/a
Type:fluorescein-labeled RNA annealed to DNA
Mol. Mass.:2134.40
Organism:n/a
Description:It is a 18-nucleotide 3-fluorescein-labeled RNA annealed to a complementary 18-nucleotide 5-Dabcyl-modified DNA.
Residue:23
Sequence:
5-GAU CUG AGC CUG GGA GCU-fluorescein-3