Reaction Details |
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Target | Dimer of Gag-Pol polyprotein [489-587,Q496K] |
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Ligand | BDBM517 |
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Substrate/Competitor | fluorogenic peptide substrate, SQNY/PIVQ |
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Meas. Tech. | Protease Inhibition Assay |
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pH | 5±n/a |
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Temperature | 298.15±n/a K |
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Ki | 0.543±0.057 nM |
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Km | 30540±4950 nM |
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kcat | 1.06±0.08 1/sec |
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Citation | Muzammil, S; Muzammil, S; Ross, P; Ross, P; Freire, E; Freire, E A major role for a set of non-active site mutations in the development of HIV-1 protease drug resistance. Biochemistry42:631-8 (2003) [PubMed] Article |
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More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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Dimer of Gag-Pol polyprotein [489-587,Q496K] |
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Name: | Dimer of Gag-Pol polyprotein [489-587,Q496K] |
Synonyms: | HIV-1 Protease | HIV-1 Protease (Q7K) |
Type: | Protein Complex |
Mol. Mass.: | n/a |
Description: | n/a |
Components: | This complex has 2 components. |
Component 1 |
Name: | Gag-Pol polyprotein [489-587,Q496K] |
Synonyms: | HIV-1 Protease (Q7K) chain A | HIV-1 Protease (Q7K) chain B | HIV-1 Protease B Subtype chain A | HIV-1 Protease B Subtype chain B | HIV-1 Protease Mutant (Q7K) | HIV-1 Protease chain A | POL_HV1H2 | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10782.21 |
Organism: | Human immunodeficiency virus type 1 |
Description: | All of the protease constructs in this study are on the wild type containing the single Q7K mutation to prevent autocatalysis.This protein has enzymatic properties similar to that of the wild type protease. |
Residue: | 99 |
Sequence: | PQVTLWKRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
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Component 2 |
Name: | Gag-Pol polyprotein [489-587,Q496K] |
Synonyms: | HIV-1 Protease (Q7K) chain A | HIV-1 Protease (Q7K) chain B | HIV-1 Protease B Subtype chain A | HIV-1 Protease B Subtype chain B | HIV-1 Protease Mutant (Q7K) | HIV-1 Protease chain A | POL_HV1H2 | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10782.21 |
Organism: | Human immunodeficiency virus type 1 |
Description: | All of the protease constructs in this study are on the wild type containing the single Q7K mutation to prevent autocatalysis.This protein has enzymatic properties similar to that of the wild type protease. |
Residue: | 99 |
Sequence: | PQVTLWKRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
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BDBM517 |
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fluorogenic peptide substrate, SQNY/PIVQ |
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Name: | fluorogenic peptide substrate, SQNY/PIVQ |
Synonyms: | n/a |
Type: | Peptide |
Mol. Mass.: | 4728.18 |
Organism: | n/a |
Description: | n/a |
Residue: | 44 |
Sequence: | Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-A
rg
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