| Reaction Details |
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 | Report a problem with these data |
| Target | Genome polyprotein |
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| Ligand | BDBM35553 |
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| Substrate/Competitor | poly(A)/oligo(U)18 |
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| Meas. Tech. | HCV RNA-Dependent RNA Polymerase Assay (IC50) and Replicon Cell-Based ELISA Assay (EC50) |
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| IC50 | 180±n/a nM |
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| EC50 | 11000±n/a nM |
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| Citation |  Ontoria, JM; Rydberg, EH; Di Marco, S; Tomei, L; Attenni, B; Malancona, S; Martin Hernando, JI; Gennari, N; Koch, U; Narjes, F; Rowley, M; Summa, V; Carroll, SS; Olsen, DB; De Francesco, R; Altamura, S; Migliaccio, G; Carfì, A Identification and Biological Evaluation of a Series of 1H-Benzo[de]isoquinoline-1,3(2H)-diones as Hepatitis C Virus NS5B Polymerase Inhibitors. J Med Chem52:5217-27 (2009) [PubMed] Article |
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| More Info.: | Get all data from this article, Solution Info, Assay Method |
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| Genome polyprotein |
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| Name: | Genome polyprotein |
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| Synonyms: | HCV NS5B Polymerase | POLG_HCVJ8 | RNA-directed RNA polymerase, C-terminally truncated form delta C21 |
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| Type: | Enzyme catalytic domain |
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| Mol. Mass.: | 330236.08 |
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| Organism: | Hepatitis C virus genotype 2b (isolate HC-J8) (HCV) |
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| Description: | P26661 |
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| Residue: | 3033 |
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| Sequence: | MSTNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRG
RRQPIPKDRRSTGKSWGKPGYPWPLYGNEGCGWAGWLLSPRGSRPTWGPTDPRHRSRNLG
RVIDTITCGFADLMGYIPVVGAPVGGVARALAHGVRVLEDGINYATGNLPGCSFSIFLLA
LLSCVTVPVSAVEVRNISSSYYATNDCSNNSITWQLTDAVLHLPGCVPCENDNGTLHCWI
QVTPNVAVKHRGALTRSLRTHVDMIVMAATACSALYVGDVCGAVMILSQAFMVSPQRHNF
TQECNCSIYQGHITGHRMAWDMMLSWSPTLTMILAYAARVPELVLEIIFGGHWGVVFGLA
YFSMQGAWAKVIAILLLVAGVDATTYSSGQEAGRTVAGFAGLFTTGAKQNLYLINTNGSW
HINRTALNCNDSLQTGFLASLFYTHKFNSSGCPERLSSCRGLDDFRIGWGTLEYETNVTN
DGDMRPYCWHYPPRPCGIVPARTVCGPVYCFTPSPVVVGTTDKQGVPTYTWGENETDVFL
LNSTRPPRGAWFGCTWMNGTGFTKTCGAPPCRIRKDYNSTIDLLCPTDCFRKHPDATYLK
CGAGPWLTPRCLVDYPYRLWHYPCTVNFTIFKARMYVGGVEHRFSAACNFTRGDRCRLED
RDRGQQSPLLHSTTEWAVLPCSFSDLPALSTGLLHLHQNIVDVQYLYGLSPALTRYIVKW
EWVILLFLLLADARICACLWMLIILGQAEAALEKLIILHSASAASANGPLWFFIFFTAAW
YLKGRVVPVATYSVLGLWSFLLLVLALPQQAYALDAAEQGELGLAILVIISIFTLTPAYK
ILLSRSVWWLSYMLVLAEAQIQQWVPPLEVRGGRDGIIWVAVILHPRLVFEVTKWLLAIL
GPAYLLKASLLRIPYFVRAHALLRVCTLVKHLAGARYIQMLLITIGRWTGTYIYDHLSPL
STWAAQGLRDLAIAVEPVVFSPMEKKVIVWGAETVACGDILHGLPVSARLGREVLLGPAD
GYTSKGWKLLAPITAYTQQTRGLLGAIVVSLTGRDKNEQAGQVQVLSSVTQTFLGTSISG
VLWTVYHGAGNKTLAGPKGPVTQMYTSAEGDLVGWPSPPGTKSLDPCTCGAVDLYLVTRN
ADVIPVRRKDDRRGALLSPRPLSTLKGSSGGPVLCSRGHAVGLFRAAVCARGVAKSIDFI
PVESLDVATRTPSFSDNSTPPAVPQSYQVGYLHAPTGSGKSTKVPAAYASQGYKVLVLNP
SVAATLGFGAYMSKAHGINPNIRTGVRTVTTGDSITYSTYGKFIADGGCAAGAYDIIICD
ECHSVDATTILGIGTVLDQAETAGVRLVVLATATPPGTVTTPHSNIEEVALGHEGEIPFY
GKAIPLAFIKGGRHLIFCHSKKKCDELAAALRGMGVNAVAYYRGLDVSVIPTQGDVVVVA
TDALMTGYTGDFDSVIDCNVAVSQIVDFSLDPTFTITTQTVPQDAVSRSQRRGRTGRGRL
GVYRYVSSGERPSGMFDSVVLCECYDAGAAWYELTPAETTVRLRAYFNTPGLPVCQDHLE
FWEAVFTGLTHIDAHFLSQTKQGGENFAYLTAYQATVCARAKAPPPSWDVMWKCLTRLKP
TLTGPTPLLYRLGAVTNEVTLTHPVTKYIATCMQADLEIMTSSWVLAGGVLAAVAAYCLA
TGCISIIGRLHLNDRVVVAPDKEILYEAFDEMEECASKAALIEEGQRMAEMLKSKIQGLL
QQATRQAQDIQPAIQSSWPKLEQFWAKHMWNFISGIQYLAGLSTLPGNPAVASMMAFSAA
LTSPLPTSTTILLNIMGGWLASQIAPPAGATGFVVSGLVGAAVGSIGLGKILVDVLAGYG
AGISGALVAFKIMSGEKPTVEDVVNLLPAILSPGALVVGVICAAILRRHVGQGEGAVQWM
NRLIAFASRGNHVAPTHYVVESDASQRVTQVLSSLTITSLLRRLHAWITEDCPVPCSGSW
LQDIWDWVCSILTDFKNWLSSKLLPKMPGIPFISCQKGYKGVWAGTGVMTTRCPCGANIS
GHVRMGTMKITGPKTCLNLWQGTFPINCYTEGPCVPKPPPNYKTAIWRVAASEYVEVTQH
GSFSYVTGLTSDNLKVPCQVPAPEFFSWVDGVQIHRFAPVPGPFFRDEVTFTVGLNSFVV
GSQLPCDPEPDTEVLASMLTDPSHITAEAAARRLARGSPPSQASSSASQLSAPSLKATCT
THKTAYDCDMVDANLFMGGDVTRIESDSKVIVLDSLDSMTEVEDDREPSVPSEYLIKRRK
FPPALPPWARPDYNPVLIETWKRPGYEPPTVLGCALPPTPQTPVPPPRRRRAKVLTQDNV
EGVLREMADKVLSPLQDNNDSGHSTGADTGGDIVQQPSDETAASEAGSLSSMPPLEGEPG
DPDLEFEPVGSAPPSEGECEVIDSDSKSWSTVSDQEDSVICCSMSYSWTGALITPCGPEE
EKLPINPLSNSLMRFHNKVYSTTSRSASLRAKKVTFDRVQVLDAHYDSVLQDVKRAASKV
SARLLTVEEACALTPPHSAKSRYGFGAKEVRSLSRRAVNHIRSVWEDLLEDQHTPIDTTI
MAKNEVFCIDPTKGGKKPARLIVYPDLGVRVCEKMALYDIAQKLPKAIMGPSYGFQYSPA
ERVDFLLKAWGSKKDPMGFSYDTRCFDSTVTERDIRTEESIYQACSLPQEARTVIHSLTE
RLYVGGPMTNSKGQSCGYRRCRASGVFTTSMGNTMTCYIKALAACKAAGIVDPVMLVCGD
DLVVISESQGNEEDERNLRAFTEAMTRYSAPPGDLPRPEYDLELITSCSSNVSVALDSRG
RRRYFLTRDPTTPITRAAWETVRHSPVNSWLGNIIQYAPTIWVRMVIMTHFFSILLAQDT
LNQNLNFEMYGAVYSVNPLDLPAIIERLHGLEAFSLHTYSPHELSRVAATLRKLGAPPLR
AWKSRARAVRASLIAQGARAAICGRYLFNWAVKTKLKLTPLPEASRLDLSGWFTVGAGGG
DIYHSVSHARPRLLLLCLLLLSVGVGIFLLPAR
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| BDBM35553 |
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| poly(A)/oligo(U)18 |
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| Name: | poly(A)/oligo(U)18 |
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| Synonyms: | n/a |
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| Type: | homopolymeric template/primer |
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| Mol. Mass.: | 358.43 |
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| Organism: | n/a |
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| Description: | UTP/3H-UTP as co-substrate. |
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| Residue: | 3 |
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| Sequence: | |
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