| Reaction Details |
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 | Report a problem with these data |
| Target | Genome polyprotein |
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| Ligand | BDBM35553 |
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| Substrate/Competitor | poly(A)/oligo(U)18 |
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| Meas. Tech. | HCV RNA-Dependent RNA Polymerase Assay (IC50) |
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| IC50 | 490±n/a nM |
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| Citation |  Ontoria, JM; Rydberg, EH; Di Marco, S; Tomei, L; Attenni, B; Malancona, S; Martin Hernando, JI; Gennari, N; Koch, U; Narjes, F; Rowley, M; Summa, V; Carroll, SS; Olsen, DB; De Francesco, R; Altamura, S; Migliaccio, G; Carfì, A Identification and Biological Evaluation of a Series of 1H-Benzo[de]isoquinoline-1,3(2H)-diones as Hepatitis C Virus NS5B Polymerase Inhibitors. J Med Chem52:5217-27 (2009) [PubMed] Article |
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| More Info.: | Get all data from this article, Solution Info, Assay Method |
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| Genome polyprotein |
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| Name: | Genome polyprotein |
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| Synonyms: | HCV NS5B Polymerase | POLG_HCVEU | RNA-directed RNA polymerase | p68 |
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| Type: | Enzyme |
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| Mol. Mass.: | 329083.72 |
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| Organism: | Hepatitis C virus genotype 6a (isolate EUHK2) (HCV) |
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| Description: | O39927 |
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| Residue: | 3018 |
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| Sequence: | MSTLPKPQRKTKRNTNRRPMDVKFPGGGQIVGGVYLLPRKGPRLGVRATRKTSERSQPRG
RRQPIPKARQPQGRHWAQPGYPWPLYGSEGCGWAGWLLSPRGSRPHWGPNDPRRRSRNLG
KVIDTLTCGFADLMWYIPVVGAPLGGVAAALAHGVRAIEDGINYATGNLPGCSFSIFLLA
LLSCLTTPASALTYGNSSGLYHLTNDCSNSSIVLEADAMILHLPGCLPCVRVGNQSTCWH
AVSPTLATPNASTPATGFRRHVDLLAGAAVVCSSLYIGDLCGSLFLAGQLFAFQPRRHWT
VQDCNCSIYTGHVTGHKMAWDMMMNWSPTTTLVLSSILRVPEICASVIFGGHWGILLAVA
YFGMAGNWLKVLAVLFLFAGVEAQTMIAHGVSQTTSGFASLLTPGAKQNIQLINTNGSWH
INRTALNCNDSLQTGFLASLFYTHKFNSSGCPERMAACKPLAEFRQGWGQITHKNVSGPS
DDRPYCWHYAPRPCEVVPARSVCGPVYCFTPSPVVVGTTDKRGNPTYTWGENETDVFMLE
SLRPPTGGWFGCTWMNSTGFTKTCGAPPCQIVPGNYNSSANELLCPTDCFRKHPEATYQR
CGSGPWVTPRCLVDYAYRLWHYPCTVNFTLHKVRMFVGGTEHRFDVACNWTRGERCELHD
RNRIEMSPLLFSTTQLSILPCSFSTMPALSTGLIHLHQNIVDVQYLYGVSTNVTSWVVKW
EYIVLMFLVLADARICTCLWLMLLISTVEAAVERLVVLNAASAAGTAGWWWAVLFLCCVW
YVKGRLVPACTYMALGMWPLLLTILALPPRAYAMDNEQAASLGAVGLLVITIFSITPMYK
KLLNCFIWWNQYFLARAEAMVHEWVPDLRVRGGRDSIILLTCLLHPQLGFEVTKILLAVL
APLYILQYSLLKVPYFVRAHILLRACLLVRRLAGGKYVQACLLRLGAWTGTFVYDHLAPL
SDWASDGLRDLAVAVEPVIFSPMEKKIITWGADTAACGDILSGLPVSARLGNLVLLGPAD
DMQRGGWKLLAPITAYAQQTRGLVGTIVTSLTGRDKNEVEGEVQVVSTDTQSFVATSING
VMWTVYHGPGFKTLAGPKGPVCQMYTNVDLDLVGWPSPPGARSLTPCNCGSSDLYLVTRE
ADVIPARRRGDSRAALLSPRPISTLKGSSGGPIMCPSGHVVGLFRAAVCTRGVAKSLDFI
PVENMETTMRSPSFTDNSTPPAVPQTYQVGYLHAPTGSGKSTRVPAAYASQGYKVLVLNP
SVAATLSFGSYMRQAYGVEPNIRTGVRTVTTGGAITYSTYGEFLADGGCSGGAYDIIICD
ECHSTDPTTVLGVGTVLDQAETAGVRLTVLPTATPPGSVTVPHPNITETALPTTGEIPFY
GKAIPLEYIKGGRHLIFCHSKKKCDELAGKLKSLGLNAVAFYRGVDVSVIPTSGDVVVCA
TDALMTGYTGDFDSVIDCNVAVTQVVDFSLDPTFSIETTTVPQDAVSRSQRRGRTGRGKP
GVYRFVSQGERPSGMFDTVVLCEAYDTGCAWYELTPSETTVRLRAYMNTPGLPVCQDHLE
FWEGVFTGLTHIDAHFLSHTKQAGENFAYLVAYQATVCARAKAPPPSWDMMWKCLIRLKP
TLTGPTPLLYRLGAVQNGVITTHPITKYIMTCMSADLEVITSTWVLVGGVLAALAAYCLS
VGCVVICGRITLTGKPAVVPDREILYQQFDEMEECSRHIPYLAEGQQIAEQFRQKVLGLL
QASAKQAEELKPAVHSAWPRVEDFWRKHMWNFVSGIQYLAGLSTLPGNPAVASLMSFTAS
LTSPLRTSQTLLLNILGGWIAAQVAPPPASTAFVVSGLAGAAVGSIRLGRVLVDVLAGYG
AGVSGALVAFKIMSGECPSTEDMVNLLPALLSPGVALVGVVCAAILRRHVGPAEGANQWM
NRLIAFASRGNHVSPTHYVPETDASKNVTQILTSLTITSLLRRLHQWVNEDTATPCATSW
LRDVWDWVCTVLSDFKVWLQAKLFPRLPGIPFLSCQAGYRGVWAGDGVCHTTCTCGAVIA
GHVKNGTMKITGPKTCSNTWHGTFPINATTTGPSTPRPAPNYQRALWRVSAEDYVEVRRL
GDCHYVVGVTAEGLKCPCQVPAPEFFTEVDGVRIHRYAPPCKPLLRDEVTFSVGLSNYAV
GSQLPCEPEPDVTVVTSMLTDPTHITAETAARRLKKGSPPSLASSSANQLSAPSLRATCT
TSQKHPEMELLQANLLWKHEMGSHIPRVQSENKVVVLDSFELYPLEYEEREISVSVECHR
QPRCKFPPVFPVWARPDNNPPFIQAWQMPGYEPPVVSGCAVAPPKPAPVPPPRRKRLVHL
DESTVSHALAQLADKVFVESSNDPGPSSDSGLSITSPVPPDPTTPEDAGSEAESYSSMPP
LEGEPGDPDLSSGSWSTVSDEDDVVCCSMSYSWTGALITPCAAEEEKLPINPLSNSLVRH
HNMVYSTTSRSASLRQKKVTFDRVQVFDQHYQDVLKEIKLRASTVQAKLLSIEEACDLTP
SHSARSKYGYGAQDVRSRASKAVDHIPSVWEGLLEDSDTPIPTTIMAKNEVFCVDPSKGG
RKPARLIVYPDLGVRVCEKMALYDVTQKLPQAVMGPAYGFQYSPNQRVEYLLKMWRSKKV
PMGFSYDTRCFDSTVTERDIRTENDIYQSCQLDPVARRVVSSLTERLYVGGPMANSKGQS
CGYRRCRASGVLPTSMGNTLTCYLKAQAACRAANIKDCDMLVCGDDLVVICESAGVQEDT
ASLRAFTDAMTRYSAPPGDAPQPTYDLELITSCSSNVSVAHEGNGKKYYYLTRDCTTPLA
RAAWETARHTPVNSWLGNIIMFAPTIWVRMVLMNHFFSILQSQEQLEKAFDFDIYGVTYS
VSPLDLPAIIQRLHGMAAFSLHGYSPVELNRVGACLRKLGVLPSRAWRHRARAVRAKLIA
QGGKAAICGKYLFNWAVKTKLKLTPLVSASKLDLSGWFVAGYDGGDIYHSVSQARPRFLL
LGLLLLTVGVGIFLLPAR
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| BDBM35553 |
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| poly(A)/oligo(U)18 |
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| Name: | poly(A)/oligo(U)18 |
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| Synonyms: | n/a |
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| Type: | homopolymeric template/primer |
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| Mol. Mass.: | 358.43 |
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| Organism: | n/a |
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| Description: | UTP/3H-UTP as co-substrate. |
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| Residue: | 3 |
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| Sequence: | |
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