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TargetHIV-1 Protease
LigandBDBM845
Substrate/CompetitorFluorogenic Peptide Substrate
Meas. Tech.Protease Inhibition Assay
pH5±n/a
Temperature303.15±n/a K
Ki 0.3±n/a nM
Citation Alterman MAndersson HOGarg NAhlsen GLovgren SClasson BDanielson UHKvarnstrom IVrang LUnge TSamuelsson BHallberg A Design and fast synthesis of C-terminal duplicated potent C(2)-symmetric P1/P1'-modified HIV-1 protease inhibitors. J Med Chem 42:3835-44 (1999) [PubMed]
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
HIV-1 Protease
Name:HIV-1 Protease
Synonyms:HIV-1 Protease, recombinant, isolate HXB2
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:HIV-1 Protease chain A
Synonyms:BRU isolated | HIV-1 Protease B Subtype Chain A | HIV-1 Protease B Subtype Chain B | LAI(Wild type) | Protease Retropepsin
Type:Enzyme Subunit
Mol. Mass.:10795.19
Organism:Human immunodeficiency virus type 1
Description:n/a
Residue:99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
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  Blast E-value cutoff:
Component 2
Name:HIV-1 Protease chain A
Synonyms:BRU isolated | HIV-1 Protease B Subtype Chain A | HIV-1 Protease B Subtype Chain B | LAI(Wild type) | Protease Retropepsin
Type:Enzyme Subunit
Mol. Mass.:10795.19
Organism:Human immunodeficiency virus type 1
Description:n/a
Residue:99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM845
Fluorogenic Peptide Substrate
Name:Fluorogenic Peptide Substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:4156.59
Organism:n/a
Description:n/a
Residue:39
Sequence:
DABCYL-gamma-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS