| Reaction Details |
|---|
 | Report a problem with these data |
| Target | Gag-Pol polyprotein [600-1027,Y780C]/[600-1155,Y780C] |
|---|
| Ligand | BDBM1530 |
|---|
| Substrate/Competitor | poly(rC):oligo(dG) |
|---|
| Meas. Tech. | HIV-1 RT Assay |
|---|
| pH | 7.8±n/a |
|---|
| Temperature | 298.15±n/a K |
|---|
| IC50 | 920±n/a nM |
|---|
| Citation |  Proudfoot, JR; Hargrave, KD; Kapadia, SR; Patel, UR; Grozinger, KG; McNeil, DW; Cullen, E; Cardozo, M; Tong, L; Kelly, TA Novel non-nucleoside inhibitors of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase. 4. 2-Substituted dipyridodiazepinones as potent inhibitors of both wild-type and cysteine-181 HIV-1 reverse transcriptase enzymes. J Med Chem38:4830-8 (1995) [PubMed] Article |
|---|
| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
|---|
| |
| Gag-Pol polyprotein [600-1027,Y780C]/[600-1155,Y780C] |
|---|
| Name: | Gag-Pol polyprotein [600-1027,Y780C]/[600-1155,Y780C] |
|---|
| Synonyms: | EC: 2.7.7.49 | HIV-1 Reverse Transcriptase Mutant (Y181C) |
|---|
| Type: | Protein Complex |
|---|
| Mol. Mass.: | 115564 |
|---|
| Description: | n/a |
|---|
| Components: | This complex has 2 components. |
|---|
| Component 1 |
| Name: | Gag-Pol polyprotein [600-1155,Y780C] |
|---|
| Synonyms: | HIV-1 Reverse Transcriptase Mutant (Y181C) Chain A | POL_HV1B1 | gag-pol |
|---|
| Type: | Enzyme Subunit |
|---|
| Mol. Mass.: | 63868.77 |
|---|
| Organism: | Human immunodeficiency virus type 1 |
|---|
| Description: | P03366[600-1155,Y780C] |
|---|
| Residue: | 556 |
|---|
| Sequence: | PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI
CQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNKGRQKVVPLTNTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGI
|
|
|
|---|
| Component 2 |
| Name: | Gag-Pol polyprotein [600-1027,Y780C] |
|---|
| Synonyms: | HIV-1 Reverse Transcriptase Mutant (Y181C) Chain B | POL_HV1B1 | gag-pol |
|---|
| Type: | Enzyme Subunit |
|---|
| Mol. Mass.: | 49908.60 |
|---|
| Organism: | Human immunodeficiency virus type 1 |
|---|
| Description: | P03366[600-1027,Y780C] |
|---|
| Residue: | 428 |
|---|
| Sequence: | PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI
CQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVKQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQ
|
|
|
|---|
| BDBM1530 |
|---|
| poly(rC):oligo(dG) |
|---|
| Name: | poly(rC):oligo(dG) |
|---|
| Synonyms: | n/a |
|---|
| Type: | RNA-DNA duplex |
|---|
| Mol. Mass.: | 358.43 |
|---|
| Organism: | n/a |
|---|
| Description: | The incorporation of Tritium-labeled [3H]dGTP onto a (poly)rC:(oligo)dG template:primer. |
|---|
| Residue: | 3 |
|---|
| Sequence: | |
|---|
Search and Browse
