| Reaction Details |
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 | Report a problem with these data |
| Target | Gag-Pol polyprotein [588-1027]/[588-1147] |
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| Ligand | BDBM1434 |
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| Substrate/Competitor | poly(rA)600:oligo(dT)10 |
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| Meas. Tech. | HIV-1 RT Assay |
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| pH | 8.3±n/a |
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| Temperature | 301.15±n/a K |
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| IC50 | 3100±n/a nM |
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| Citation |  Romero, DL; Olmsted, RA; Poel, TJ; Morge, RA; Biles, C; Keiser, BJ; Kopta, LA; Friis, JM; Hosley, JD; Stefanski, KJ; Wishka, DG; Evans, DB; Morris, J; Stehle, RG; Sharma, SK; Yagi, Y; Voorman, RL; Adams, WJ; Tarpley, WG; Thomas, RC Targeting delavirdine/atevirdine resistant HIV-1: identification of (alkylamino)piperidine-containing bis(heteroaryl)piperazines as broad spectrum HIV-1 reverse transcriptase inhibitors. J Med Chem39:3769-89 (1996) [PubMed] Article |
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| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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| Gag-Pol polyprotein [588-1027]/[588-1147] |
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| Name: | Gag-Pol polyprotein [588-1027]/[588-1147] |
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| Synonyms: | EC: 2.7.7.49 | HIV-1 Reverse Transcriptase |
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| Type: | Protein Complex |
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| Mol. Mass.: | 115684 |
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| Description: | n/a |
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| Components: | This complex has 2 components. |
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| Component 1 |
| Name: | Gag-Pol polyprotein [588-1147] |
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| Synonyms: | HIV-1 Reverse Transcriptase Chain A | POL_HV1H2 | gag-pol |
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| Type: | Enzyme Subunit |
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| Mol. Mass.: | 64486.41 |
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| Organism: | Human immunodeficiency virus type 1 |
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| Description: | P04585[588-1147] |
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| Residue: | 560 |
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| Sequence: | PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGIRKVL
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| Component 2 |
| Name: | Gag-Pol polyprotein [588-1027] |
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| Synonyms: | HIV-1 Reverse Transcriptase Chain B | POL_HV1H2 | gag-pol |
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| Type: | Enzyme Subunit |
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| Mol. Mass.: | 51337.13 |
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| Organism: | Human immunodeficiency virus type 1 |
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| Description: | P04585[588-1027] |
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| Residue: | 440 |
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| Sequence: | PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETF
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| BDBM1434 |
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| poly(rA)600:oligo(dT)10 |
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| Name: | poly(rA)600:oligo(dT)10 |
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| Synonyms: | n/a |
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| Type: | RNA-DNA duplex |
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| Mol. Mass.: | 358.43 |
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| Organism: | n/a |
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| Description: | The incorporation of thymidine triphosphate with [methyl-3H]thymidine 5 -triphosphate onto a poly(rA)600:oligo(dT)10 template:primer. |
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| Residue: | 3 |
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| Sequence: | |
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