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TargetGag-Pol polyprotein [588-1127,Y768C]/[588-1147,Y768C]
LigandBDBM1317
Substrate/Competitorpoly(rA)600:oligo(dT)10
Meas. Tech.HIV-1 RT Assay
IC50>60000±n/a nM
Citation Romero, DL; Olmsted, RA; Poel, TJ; Morge, RA; Biles, C; Keiser, BJ; Kopta, LA; Friis, JM; Hosley, JD; Stefanski, KJ; Wishka, DG; Evans, DB; Morris, J; Stehle, RG; Sharma, SK; Yagi, Y; Voorman, RL; Adams, WJ; Tarpley, WG; Thomas, RC Targeting delavirdine/atevirdine resistant HIV-1: identification of (alkylamino)piperidine-containing bis(heteroaryl)piperazines as broad spectrum HIV-1 reverse transcriptase inhibitors. J Med Chem39:3769-89 (1996) [PubMed]  Article
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Gag-Pol polyprotein [588-1127,Y768C]/[588-1147,Y768C]
Name:Gag-Pol polyprotein [588-1127,Y768C]/[588-1147,Y768C]
Synonyms:EC: 2.7.7.49 | HIV-1 Reverse Transcriptase Mutant (Y181C)
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [588-1147,Y768C]
Synonyms:HIV-1 Reverse Transcriptase Mutant (Y181C) Chain A | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:64426.38
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1147,Y768C]
Residue:560
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
CQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGIRKVL
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Component 2
Name:Gag-Pol polyprotein [588-1127,Y768C]
Synonyms:HIV-1 Reverse Transcriptase Mutant (Y181C) Chain B | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:51277.10
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1127,Y768C]
Residue:440
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
CQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETF
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BDBM1317
poly(rA)600:oligo(dT)10
Name:poly(rA)600:oligo(dT)10
Synonyms:n/a
Type:RNA-DNA duplex
Mol. Mass.:358.43
Organism:n/a
Description:The incorporation of thymidine triphosphate with [methyl-3H]thymidine 5 -triphosphate onto a poly(rA)600:oligo(dT)10 template:primer.
Residue:3
Sequence:
n/a