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TargetGag-Pol polyprotein [588-1127,L687I]/[588-1147,L687I]
LigandBDBM1434
Substrate/Competitorpoly(rC)-oligo(dG)(12-18)
Meas. Tech.HIV RT Assay
pH7.8±n/a
Temperature310.15±n/a K
IC50 3500±180 nM
Citation Artico, M; Silvestri, R; Massa, S; Loi, AG; Corrias, S; Piras, G; La Colla, P 2-Sulfonyl-4-chloroanilino moiety: a potent pharmacophore for the anti-human immunodeficiency virus type 1 activity of pyrrolyl aryl sulfones. J Med Chem39:522-30 (1996) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Gag-Pol polyprotein [588-1127,L687I]/[588-1147,L687I]
Name:Gag-Pol polyprotein [588-1127,L687I]/[588-1147,L687I]
Synonyms:EC: 2.7.7.49 | HIV-1 Reverse Transcriptase Mutant (L100I)
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [588-1147,L687I]
Synonyms:HIV-1 Reverse Transcriptase Mutant (L100I) Chain A | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:64486.41
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1147,L687I]
Residue:560
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGIKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGIRKVL
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Component 2
Name:Gag-Pol polyprotein [588-1127,L687I]
Synonyms:HIV-1 Reverse Transcriptase Mutant (L100I) Chain B | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:51337.13
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1127,L687I]
Residue:440
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGIKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETF
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BDBM1434
poly(rC)-oligo(dG)(12-18)
Name:poly(rC)-oligo(dG)(12-18)
Synonyms:n/a
Type:RNA/DNA duplex
Mol. Mass.:358.43
Organism:n/a
Description:The incorporation of Tritium-labeled dGTP onto a poly(rC)-oligo(dG)(12-18) template-primer
Residue:3
Sequence:
n/a