| Reaction Details |
|---|
 | Report a problem with these data |
| Target | Gag-Pol polyprotein [588-1027,K690N]/[588-1147,K690N] |
|---|
| Ligand | BDBM2483 |
|---|
| Substrate/Competitor | poly(rC)-oligo(dG)(12-18) |
|---|
| Meas. Tech. | HIV-1 RT Assay |
|---|
| pH | 7.8±n/a |
|---|
| Temperature | 310.15±n/a K |
|---|
| IC50 | 3400±300 nM |
|---|
| Citation |  Silvestri, R; De Martino, G; La Regina, G; Artico, M; Massa, S; Vargiu, L; Mura, M; Loi, AG; Marceddu, T; La Colla, P Novel indolyl aryl sulfones active against HIV-1 carrying NNRTI resistance mutations: synthesis and SAR studies. J Med Chem46:2482-93 (2003) [PubMed] Article |
|---|
| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
|---|
| |
| Gag-Pol polyprotein [588-1027,K690N]/[588-1147,K690N] |
|---|
| Name: | Gag-Pol polyprotein [588-1027,K690N]/[588-1147,K690N] |
|---|
| Synonyms: | EC: 2.7.7.49 | HIV-1 Reverse Transcriptase Mutant (K103N) |
|---|
| Type: | Protein Complex |
|---|
| Mol. Mass.: | n/a |
|---|
| Description: | n/a |
|---|
| Components: | This complex has 2 components. |
|---|
| Component 1 |
| Name: | Gag-Pol polyprotein [588-1147,K690N] |
|---|
| Synonyms: | HIV-1 Reverse Transcriptase Mutant (K103N) Chain A | POL_HV1H2 | gag-pol |
|---|
| Type: | Enzyme Subunit |
|---|
| Mol. Mass.: | 64471.33 |
|---|
| Organism: | Human immunodeficiency virus type 1 |
|---|
| Description: | P04585[588-1147,K690N] |
|---|
| Residue: | 560 |
|---|
| Sequence: | PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKNKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGIRKVL
|
|
|
|---|
| Component 2 |
| Name: | Gag-Pol polyprotein [588-1027,K690N] |
|---|
| Synonyms: | HIV-1 Reverse Transcriptase Mutant (K103N) Chain B | POL_HV1H2 | gag-pol |
|---|
| Type: | Enzyme Subunit |
|---|
| Mol. Mass.: | 51280.01 |
|---|
| Organism: | Human immunodeficiency virus type 1 |
|---|
| Description: | P04585[588-1027,K690N] |
|---|
| Residue: | 440 |
|---|
| Sequence: | PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKNKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYNLEKEPIVGAETF
|
|
|
|---|
| BDBM2483 |
|---|
| poly(rC)-oligo(dG)(12-18) |
|---|
| Name: | poly(rC)-oligo(dG)(12-18) |
|---|
| Synonyms: | n/a |
|---|
| Type: | RNA/DNA duplex |
|---|
| Mol. Mass.: | 358.43 |
|---|
| Organism: | n/a |
|---|
| Description: | The incorporation of Tritium-labeled dGTP onto a poly(rC)-oligo(dG)(12-18) template-primer
|
|---|
| Residue: | 3 |
|---|
| Sequence: | |
|---|
Search and Browse
