| Reaction Details |
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 | Report a problem with these data |
| Target | Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform |
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| Ligand | BDBM2681 |
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| Substrate/Competitor | Phosphorylase b |
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| Meas. Tech. | Phosphorylase Kinasse Assay |
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| pH | 7±n/a |
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| Temperature | 303.15±n/a K |
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| IC50 | 1700±200 nM |
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| Citation |  Toullec, D; Pianetti, P; Coste, H; Bellevergue, P; Grand-Perret, T; Ajakane, M; Baudet, V; Boissin, P; Boursier, E; Loriolle, F The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J Biol Chem266:15771-81 (1991) [PubMed] |
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| More Info.: | Get all data from this article, Inhibition_Run data, Solution Info, Assay Method |
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| Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform |
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| Name: | Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform |
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| Synonyms: | KPB1_RABIT | PHKA1 | Phosphorylase Kinase |
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| Type: | Enzyme Complex |
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| Mol. Mass.: | 138403.25 |
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| Organism: | Oryctolagus cuniculus (rabbit) |
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| Description: | The enzyme is a hexadecamer of four types of subunits:alpha(4)beta(4)gamma(4)delta(4) isolated from skeletal muscle of rabbits. |
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| Residue: | 1237 |
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| Sequence: | MRSRSNSGVRLDSYARLVQQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLA
YRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKT
CATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTAD
FGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQS
ILNSLLPRASTSKEVDASLLSVISFPAFAVEDSKLVEITKQEIITKLQGRYGCCRFLRDG
YKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLI
KGKNGVPLLPELYSVPPDKVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAP
GEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGINVETIAEVYPIRVQPARILS
HIYSSLGCNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNKMIV
EMLRTDLSYLCSRWRMTGQPTITFPISQTMLDEDGTSLNSSILAALRKMQDGYFGGARIQ
TGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDNYDELESGDWMDGYNSTSTARCGDEV
ARYLDHLLAHTAPHPKLAPASQKGGLNRFRAAVQTTCDLMSLVTKAKELHVQNVHMYLPT
KLFQASRPSLNLLDSSHPSQEDQVPTVRVEVHLPRDQSGEVDFQALVLQLKETSSLQEQA
DILYMLYTMKGPDWDTELYEEGSATVRELLTELYGKVGKIRHWGLIRYISGILRKKVEAL
DEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEALTRLIEEACEGDMNISILTQEIMVY
LAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHH
ILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSIS
TESQPNGGHSLGADLMSPSFLSPGTSVTPSSGSFPGHHTSKDSRQGQWQRRRRLDGALNR
VPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQL
LVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICT
LLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ
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| BDBM2681 |
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| Phosphorylase b |
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| Name: | Phosphorylase b |
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| Synonyms: | n/a |
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| Type: | Peptide |
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| Mol. Mass.: | 1157.29 |
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| Organism: | n/a |
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| Description: | 250 uM [gamma-32P]ATP as co-substrate. |
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| Residue: | 12 |
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| Sequence: | |
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