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TargetcAMP-dependent protein kinase catalytic subunit alpha
LigandBDBM2579
Substrate/Competitorhistone (type Vs)
Meas. Tech.PKA assay
pH8.5±n/a
Temperature310.15±n/a K
IC50 121±18 nM
Citation Bit, RA; Davis, PD; Elliott, LH; Harris, W; Hill, CH; Keech, E; Kumar, H; Lawton, G; Maw, A; Nixon, JS Inhibitors of protein kinase C. 3. Potent and highly selective bisindolylmaleimides by conformational restriction. J Med Chem36:21-9 (1993) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
cAMP-dependent protein kinase catalytic subunit alpha
Name:cAMP-dependent protein kinase catalytic subunit alpha
Synonyms:KAPCA_BOVIN | PKA C-alpha | PKC | PRKACA | Protein Kinase C | Protein Kinase C, bovine brain | cAMP-Dependent Protein Kinase (PKA) | cAMP-dependent Protein Kinase, bovine heart | cAMP-dependent protein kinase A | cAMP-dependent protein kinase alpha-catalytic subunit | cAMP-dependent protein kinase, alpha-catalytic subunit
Type:Enzyme Complex
Mol. Mass.:40627.77
Organism:Bos taurus (bovine)
Description:The PKA holoenzyme purified from bovine heart, exists as an inactive tetrameric complex, which consists of a regulatory dimer associated with two catalytic subunits. It requires cAMP to activate the enzymatic reaction.
Residue:351
Sequence:
MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWENPAQNTAHLDQFERIKTLGTGSFGRVML
VKHMETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMV
MEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGY
IQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFF
ADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFAT
TDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF
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  Blast E-value cutoff:
BDBM2579
histone (type Vs)
Name:histone (type Vs)
Synonyms:n/a
Type:Other Protein Type
Mol. Mass.:358.43
Organism:n/a
Description:11 uM [gamma-32P]ATP as co-substrate.
Residue:3
Sequence:
n/a