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TargetGag-Pol polyprotein [588-1027]/[588-1147]
LigandBDBM2483
Substrate/Competitor(poly)rA:(oligo)dT(12-18)
Meas. Tech.HIV-1 RT Assay
pH8.2±n/a
Temperature310.15±n/a K
IC50 48±n/a nM
Citation Patel, M; McHugh, RJ; Cordova, BC; Klabe, RM; Erickson-Viitanen, S; Trainor, GL; Ko, SS Synthesis and evaluation of benzoxazinones as HIV-1 reverse transcriptase inhibitors. Analogs of Efavirenz (SUSTIVA). Bioorg Med Chem Lett9:3221-4 (1999) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Gag-Pol polyprotein [588-1027]/[588-1147]
Name:Gag-Pol polyprotein [588-1027]/[588-1147]
Synonyms:EC: 2.7.7.49 | HIV-1 Reverse Transcriptase
Type:Protein Complex
Mol. Mass.:115684
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [588-1147]
Synonyms:HIV-1 Reverse Transcriptase Chain A | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:64486.41
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1147]
Residue:560
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGIRKVL
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Component 2
Name:Gag-Pol polyprotein [588-1027]
Synonyms:HIV-1 Reverse Transcriptase Chain B | POL_HV1H2 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:51337.13
Organism:Human immunodeficiency virus type 1
Description:P04585[588-1027]
Residue:440
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETF
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BDBM2483
(poly)rA:(oligo)dT(12-18)
Name:(poly)rA:(oligo)dT(12-18)
Synonyms:n/a
Type:RNA-DNA duplex
Mol. Mass.:358.43
Organism:n/a
Description:The incorporation of radiolabeled dTTP onto a (poly)rA:(oligo)dT(12-18) template:primer
Residue:3
Sequence:
n/a