| Reaction Details |
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 | Report a problem with these data |
| Target | Polyunsaturated fatty acid 5-lipoxygenase |
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| Ligand | BDBM106774 |
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| Substrate/Competitor | n/a |
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| Meas. Tech. | IC50 Assay |
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| pH | 7.3±n/a |
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| Temperature | 298.15±n/a K |
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| IC50 | 3.1e+4±n/a nM |
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| Comments | extracted |
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| Citation |  Mascayano, C; Espinosa, V; Sepúlveda-Boza, S; Hoobler, EK; Perry, S; Diaz, G; Holman, TR Enzymatic Studies of Isoflavonoids as Selective and Potent Inhibitors of Human Leukocyte 5-Lipo-Oxygenase. Chem Biol Drug Des86:114-21 (2015) [PubMed] Article |
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| More Info.: | Get all data from this article, Assay Method |
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| Polyunsaturated fatty acid 5-lipoxygenase |
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| Name: | Polyunsaturated fatty acid 5-lipoxygenase |
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| Synonyms: | 5-LO | 5-Lipo-oxygenase (5-LOX) | 5-Lipoxygenase (5-LO) | 5-Lipoxygenase (LOX) | 5-Lipoygenase | 5-lipoxygenase/FLAP | ALOX5 | Arachidonate 5-lipoxygenase | LOG5 | LOX5_HUMAN |
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| Type: | Enzyme |
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| Mol. Mass.: | 77972.74 |
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| Organism: | Homo sapiens (Human) |
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| Description: | Recombinant protein was purified from E. coli lysate. After ammonium sulfate precipitation and subsequent steps, the supernatant (S100) was used for 5-LO activity assay.
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| Residue: | 674 |
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| Sequence: | MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDE
ELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLA
RDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVL
NYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNG
CNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDP
CTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDF
HVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECG
LFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWE
AIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYL
TVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCW
HLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPY
YYLSPDRIPNSVAI
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| BDBM106774 |
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| n/a |
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| Name | BDBM106774 |
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| Synonyms: | 3‐(4‐chlorophenyl)‐6,7‐dihydroxy‐4H‐chromen‐4‐one | 6,7-Dihydroxy-4′-chloro-isoflavone (1a) | IR-5 |
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| Type | Small organic molecule |
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| Emp. Form. | C15H9ClO4 |
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| Mol. Mass. | 288.683 |
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| SMILES | Oc1cc2occ(-c3ccc(Cl)cc3)c(=O)c2cc1O |
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| Structure | 
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