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TargetCyclin-A2 [171-432]/Cyclin-dependent kinase 2
LigandBDBM5554
Substrate/CompetitorHistone H1
Meas. Tech.Kinase Inhibition Assay
IC50 300±n/a nM
Citation Hardcastle, IRArris, CEBentley, JBoyle, FTChen, YCurtin, NJEndicott, JAGibson, AEGolding, BTGriffin, RJJewsbury, PMenyerol, JMesguiche, VNewell, DRNoble, MEPratt, DJWang, LZWhitfield, HJ N2-substituted O6-cyclohexylmethylguanine derivatives: potent inhibitors of cyclin-dependent kinases 1 and 2. J Med Chem47:3710-22 (2004) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Cyclin-A2 [171-432]/Cyclin-dependent kinase 2
Name:Cyclin-A2 [171-432]/Cyclin-dependent kinase 2
Synonyms:CDK2/cyclin A3 | Cyclin-Dependent Kinase 2 (CDK2) | p33cdk2/cyclin A3
Type:Protein Complex
Mol. Mass.:n/a
Description:Binary complex of CDK2-cyclin A3 was formed by adding cyclin A3 in slight molar excess to the CDK2 subunit and purified by Superdex 75 gel filtration chromatography.
Components:This complex has 2 components.
Component 1
Name:Cyclin-dependent kinase 2
Synonyms:CDK2 | CDK2-Kinase | CDK2_HUMAN | CDKN2 | Cell division protein kinase 2 | Cyclin-dependent kinase 2 (CDK2) | Protein cereblon/Cyclin-dependent kinase 2 | p33 protein kinase
Type:Enzyme Subunit
Mol. Mass.:33938.17
Organism:Homo sapiens (Human)
Description:P24941
Residue:298
Sequence:
MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNH
PNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHS
HRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYY
STAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSF
PKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
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Component 2
Name:Cyclin-A2 [171-432]
Synonyms:CCN1 | CCNA | CCNA2 | CCNA2_HUMAN | cyclin A2
Type:Enzyme Subunit
Mol. Mass.:30018.91
Organism:Homo sapiens (Human)
Description:cyclin A2 is a c-terminal cyclin A fragment encoding residues 171-432.
Residue:262
Sequence:
SVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNE
TLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQ
VLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKY
LPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIR
EKYKNSKYHGVSLLNPPETLNL
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BDBM5554
Histone H1
Name:Histone H1
Synonyms:Sigma Type IIIS
Type:Other Protein Type
Mol. Mass.:358.43
Organism:Bos taurus (bovine)
Description:12.5 uM ATP as co-substrate.
Residue:3
Sequence:
n/a