Reaction Details |
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Target | Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B |
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Ligand | BDBM5566 |
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Substrate/Competitor | Histone H1 |
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Meas. Tech. | Kinase Inhibition Assay |
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pH | 7.5±n/a |
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Temperature | 303.15±n/a K |
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Ki | 2500±400 nM |
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Comments | To obtain Ki value, the assay was performed in the absence of inhibitor and at two fixed inhibitor concentrations, 5 and 10 uM, with ATP concentrations ranging from 6.25 to 800 uM. Data were fitted to the Michaelis-Menten equation using unweighted nonlinear least squares regression. |
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Citation | Arris, CE; Boyle, FT; Calvert, AH; Curtin, NJ; Endicott, JA; Garman, EF; Gibson, AE; Golding, BT; Grant, S; Griffin, RJ; Jewsbury, P; Johnson, LN; Lawrie, AM; Newell, DR; Noble, ME; Sausville, EA; Schultz, R; Yu, W Identification of novel purine and pyrimidine cyclin-dependent kinase inhibitors with distinct molecular interactions and tumor cell growth inhibition profiles. J Med Chem43:2797-804 (2000) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B |
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Name: | Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B |
Synonyms: | CDK1/Cyclin B | Cyclin-Dependent Kinase 1 (CDK1) | p34cdc2/Cyclin B |
Type: | Protein Complex |
Mol. Mass.: | n/a |
Description: | p34cdc2/cyclin B was purified from M-phase starfish oocytes by affinity chromatography. |
Components: | This complex has 2 components. |
Component 1 |
Name: | Cyclin-dependent kinase 1 |
Synonyms: | CDK1_ORYLA | Cell division control protein 2 (cdc2) | P34 protein kinase | cdc2 | cdk1 |
Type: | Enzyme Subunit |
Mol. Mass.: | 34693.35 |
Organism: | Marthasterias glacialis (starfish) |
Description: | Q9DGD3 {empty hard to find) |
Residue: | 303 |
Sequence: | MEDYVKIEKIGEGTYGVVYKGRHKSTGQVVAMKKIRLESEEEGVPSTAVREVSLLQELKH
PNVVRLLDVLMQESRLYLIFEFLSMDLKKYLDSIPSGQYMDPMLVKSYLYQILEGIYFCH
RRRVLHRDLKPQNLLIDNKGVIKLADFGLARAFGVPVRVYTHEVVTLWYRAPEVLLGSPR
YSTPVDVWSTGTIFAELATKKPLFHGDSEIDQLFRIFRTLGTPNNDVWPDVESLPDYKNT
FPKWKEGSLSSMVKNLDKNGLDLLAKMLIYNPPKRISAREAMTHPYFDDLDKSTLPAACI
NGV
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Component 2 |
Name: | G2/mitotic-specific cyclin-B |
Synonyms: | CCNB_MARGL | Cyclin B | G2/mitotic-specific cyclin-B1 |
Type: | Enzyme Subunit |
Mol. Mass.: | 43790.28 |
Organism: | Marthasterias glacialis (starfish) |
Description: | n/a |
Residue: | 388 |
Sequence: | MLNGENVDSRIMGKVATRASSKGVKSTLGTRGALENISNVARNNLQAGAKKELVKAKRGM
TKSKATSSLQSVMGLNVEPMEKAKPQSPEPMDMSEINSALEAFSQNLLEGVEDIDKNDFD
NPQLCSEFVNDIYQYMRKLEREFKVRTDYMTIQEITERMRSILIDWLVQVHLRFHLLQET
LFLTIQILDRYLEVQPVSKNKLQLVGVTSMLIAAKYEEMYPPEIGDFVYITDNAYTKAQI
RSMECNILRRLDFSLGKPLCIHFLRRNSKAGGVDGQKHTMAKYLMELTLPEYAFVPYDPS
EIAAAALCLSSKILEPDMEWGTTLVHYSAYSEDHLMPIVQKMALVLKNAPTAKFQAVRKK
YSSAKFMNVSTISALTSSTVMDLADQMC
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BDBM5566 |
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Histone H1 |
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Name: | Histone H1 |
Synonyms: | Sigma Type IIIS |
Type: | Other Protein Type |
Mol. Mass.: | 358.43 |
Organism: | Bos taurus (bovine) |
Description: | 12.5 uM ATP as co-substrate. |
Residue: | 3 |
Sequence: | |