| Reaction Details |
|---|
 | Report a problem with these data |
| Target | Trans-sialidase [3-643,D247A] |
|---|
| Ligand | BDBM217358 |
|---|
| Substrate/Competitor | n/a |
|---|
| Meas. Tech. | Diffusion Ordered Spectroscopy (DOSY) |
|---|
| Temperature | 284.15±n/a K |
|---|
| Kd | 1.07e+5± 2e+3 nM |
|---|
| Comments | extracted |
|---|
| Citation |  Oliveira, IA; Gonçalves, AS; Neves, JL; von Itzstein, M; Todeschini, AR Evidence of ternary complex formation in Trypanosoma cruzi trans-sialidase catalysis. J Biol Chem289:423-36 (2014) [PubMed] Article |
|---|
| More Info.: | Get all data from this article, Assay Method |
|---|
| |
| Trans-sialidase [3-643,D247A] |
|---|
| Name: | Trans-sialidase [3-643,D247A] |
|---|
| Synonyms: | trans-Sialidase (TS D247A) |
|---|
| Type: | Enzyme |
|---|
| Mol. Mass.: | 70266.23 |
|---|
| Organism: | Trypanosoma cruzi |
|---|
| Description: | T. cruzi TS D247A mutant |
|---|
| Residue: | 641 |
|---|
| Sequence: | APGSSRVELFKRKNSTVPFEDKAGKVTERVVHSFRLPALVNVDGVMVAIADARYDTSNDN
SLIDTVAKYSVDDGETWETQIAIKNSRVSSVSRVVDPTVIVKGNKLYVLVGSYYSSRSYW
SSHGDARDWDILLAVGEVTKSIAGGKITASIKWGSPVSLKKFFPAEMEGMHTNQFLGGAG
VAIVASNGNLVYPVQVTNKRKQVFSKIFYSEDDGKTWKFGKGRSDFGCSEPVALEWEGKL
IINTRVAWKRRLVYESSDMGNTWVEAVGTLSRVWGPSPKSDHPGSQSSFTAVTIEGMRVM
LFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQVSIGDENSAYSSVLYKDDKLYCLHEINT
DEVYSLVFARLVGELRIIKSVLRSWKNWDSHLSSICTPADPAASSSESGCGPAVTTVGLV
GFLSGNASQNVWEDAYRCVNASTANAERVRNGLKFAGVGGGALWPVSQQGQNQRYRFANH
AFTLVASVTIHEAPRAASPLLGASLDSSGGKKLLGLSYDEKHQWQPIYGSTPVTPTGSWE
TGKRYHVVLTVANKIGSVYIDGELLEGSGQTVVPDGRTPDISHFYVGGYGRSDMPTISHV
TVNNVLLYNRQLNTEEIRTLFLSQDLIGTEAHMDSSSDTSA
|
|
|
|---|
| BDBM217358 |
|---|
| n/a |
|---|
| Name | BDBM217358 |
|---|
| Synonyms: | α2,3'-sialyllactose | 3-SL |
|---|
| Type | Small organic molecule |
|---|
| Emp. Form. | C23H39NO19 |
|---|
| Mol. Mass. | 633.5511 |
|---|
| SMILES | CC(=O)NC1C(O)CC(OC2C(O)C(CO)OC(OC3C(CO)OC(O)C(O)C3O)C2O)(OC1[C@H](O)[C@H](O)CO)C(O)=O |r| |
|---|
| Structure | 
|
|---|
Search and Browse
