| Reaction Details |
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 | Report a problem with these data |
| Target | (S)-6-hydroxynicotine oxidase [K287M] |
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| Ligand | BDBM217378 |
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| Substrate/Competitor | n/a |
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| Meas. Tech. | Steady-State Kinetic Assay |
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| pH | 8±0 |
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| Temperature | 571.3±0 K |
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| Kd | 6.1e+4± 1.0e+4 nM |
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| Citation |  Fitzpatrick, PF; Chadegani, F; Zhang, S; Dougherty, V Mechanism of Flavoprotein l-6-Hydroxynicotine Oxidase: pH and Solvent Isotope Effects and Identification of Key Active Site Residues. Biochemistry56:869-875 (2017) [PubMed] Article |
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| More Info.: | Get all data from this article, Assay Method |
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| (S)-6-hydroxynicotine oxidase [K287M] |
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| Name: | (S)-6-hydroxynicotine oxidase [K287M] |
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| Synonyms: | 6-hlno | HLNO_PAENI | L-6-hydroxynicotine oxidase (LHNO K287M) |
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| Type: | Enzyme |
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| Mol. Mass.: | 46319.23 |
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| Organism: | Arthrobacter nicotinovorans |
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| Description: | A. nicotinovorans LHNO K287M mutant |
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| Residue: | 425 |
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| Sequence: | MYDAIVVGGGFSGLKAARDLTNAGKKVLLLEGGERLGGRAYSRESRNVPGLRVEIGGAYL
HRKHHPRLAAELDRYGIPTAAASEFTSFRHRLGPTAVDQAFPIPGSEAVAVEAATYTLLR
DAHRIDLEKGLENQDLEDLDIPLNEYVDKLDLPPVSRQFLLAWAWNMLGQPADQASALWM
LQLVAAHHYSILGVVLSLDEVFSNGSADLVDAMSQEIPEIRLQTVVTGIDQSGDVVNVTV
KDGHAFQAHSVIVATPMNTWRRIVFTPALPERRRSVIEEGHGGQGLMILIHVRGAEAGIE
CVGDGIFPTLYDYCEVSESERLLVAFTDSGSFDPTDIGAVKDAVLYYLPEVEVLGIDYHD
WIADPLFEGPWVAPRVGQFSRVHKELGEPAGRIHFVGSDVSLEFPGYIEGALETAECAVN
AILHS
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| BDBM217378 |
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| n/a |
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| Name | BDBM217378 |
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| Synonyms: | (S)-6-hydroxynicotine | 5-[(2S)-1-methylpyrrolidin-2-yl]-1H-pyridin-2-one |
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| Type | Small organic molecule |
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| Emp. Form. | C10H14N2O |
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| Mol. Mass. | 178.231 |
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| SMILES | CN1CCC[C@H]1c1ccc(=O)[nH]c1 |
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| Structure | 
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