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TargetGlycogen synthase kinase-3 beta
LigandBDBM7360
Substrate/CompetitorGSK-3 Peptide Substrate
Meas. Tech.Kinase Inhibition Assay
Temperature303.15±n/a K
IC50 400±n/a nM
Commentsextracted
Citation Mettey, Y; Gompel, M; Thomas, V; Garnier, M; Leost, M; Ceballos-Picot, I; Noble, M; Endicott, J; Vierfond, JM; Meijer, L Aloisines, a new family of CDK/GSK-3 inhibitors. SAR study, crystal structure in complex with CDK2, enzyme selectivity, and cellular effects. J Med Chem46:222-36 (2003) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Glycogen synthase kinase-3 beta
Name:Glycogen synthase kinase-3 beta
Synonyms:GSK-3, beta | GSK3B_RAT | Glycogen Synthase Kinase-3, beta | Gsk3b
Type:Enzyme
Mol. Mass.:46754.43
Organism:Rattus norvegicus (rat)
Description:The rat GSK-3 beta cDNA was inserted into the baculovirus vector. The protein was purified from virus-infected sf9 insect cells
Residue:420
Sequence:
MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTK
VIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSG
EKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHR
DIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDM
WSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHP
WTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALF
NFTTQELSSNPPLATILIPPHARIQAAASPPANATAASDTNAGDRGQTNNAASASASNST
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  Blast E-value cutoff:
BDBM7360
GSK-3 Peptide Substrate
Name:GSK-3 Peptide Substrate
Synonyms:GS-1 Peptide | Phospho-GS2 Substrate Peptide
Type:Peptide
Mol. Mass.:3045.17
Organism:n/a
Description:n/a
Residue:27
Sequence:
YRRAAVPPSPSLSRHSSPHQS(PO4)EDEEE