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TargetGlycogen synthase kinase-3 beta
LigandBDBM7403
Substrate/CompetitorGSK-3 Peptide Substrate
Meas. Tech.Kinase Inhibition Assay
Temperature303.15±n/a K
IC50 30±n/a nM
Commentsextracted
Citation Polychronopoulos, PMagiatis, PSkaltsounis, ALMyrianthopoulos, VMikros, ETarricone, AMusacchio, ARoe, SMPearl, LLeost, MGreengard, PMeijer, L Structural basis for the synthesis of indirubins as potent and selective inhibitors of glycogen synthase kinase-3 and cyclin-dependent kinases. J Med Chem47:935-46 (2004) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Glycogen synthase kinase-3 beta
Name:Glycogen synthase kinase-3 beta
Synonyms:GSK-3 alpha/beta | Glycogen Synthase Kinase-3, beta
Type:Enzyme Mixture
Mol. Mass.:48062.20
Organism:Porcine
Description:Purification of GSK-3 alpha and GSK-3 beta from Porcine Brain on Axin-Sepharose. Porcine brains were obtained from a local slaughter and directly homogenized and processed for affinity chromatography.
Residue:433
Sequence:
MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTK
VIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSG
EKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHR
DIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDV
WSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHP
WTKDSSGTGHFTSGVRVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPN
VKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPSNATAASDTNAGDRGQ
TNNTASASASNST
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  Blast E-value cutoff:
BDBM7403
GSK-3 Peptide Substrate
Name:GSK-3 Peptide Substrate
Synonyms:GS-1 Peptide | Phospho-GS2 Substrate Peptide
Type:Peptide
Mol. Mass.:3045.17
Organism:n/a
Description:n/a
Residue:27
Sequence:
YRRAAVPPSPSLSRHSSPHQS(PO4)EDEEE