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Assay Method Information

Assay Name:  Kinase Inhibition SPA Assay
Description:  The biochemical activity of compounds was determined by incubation with specific enzyme and substrate in the presence 2.5 uM ATP/ [gamma-32P] ATP. After incubation, the reaction was stopped and the phosphorylated substrate was separated from nonincorporated radioactive ATP using Streptavidin-coated SPA beads (Amersham Pharmacia), counted using TopCount (Packard) to measure substrate-incorporated phosphate.
Affinity data for this assay
 

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Last update November 1, 2007
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