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Assay Method Information |
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| | Fluorescence Peptide Cleavage Assay |
| Description: | Fluorescence peptide cleavage assay was performed in a 96-well plate. Each reaction contained MAPKKide, LF, and the small-molecule inhibitor. The C-terminally linked fluorophore of MAPKKide was a FITC, and the acceptor chromophore was 4-([4-(dimethylamino)-phenyl] azo) benzoic acid (DABCYL). Cleavage of the substrate by LF released the fluorophore and full fluorescence was restored. The reaction was examined by using a fluorescent plate reader at excitation and emission wavelength at 485 and 590 nm. |
| Affinity data for this assay | |
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