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| | Measurement of FBSAChE/EqBuChE Inhibitory Activity |
| Description: | Inhibition of enzyme activity was measured over a substrate concentration range of 0.01-30 mM and at least six inhibitor concentrations to determine competitive and noncompetitive inhibitors. Plots of initial velocities versus substrate concentrations at a series of inhibitor concentrations were analyzed by nonlinear least-squares methods to determine the values of Km and Vmax. Nonlinear regression analysis of the plots of Vmax/Km values versus [THA-An] was used for the determination of Ki value |
| Affinity data for this assay | |
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