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Assay Method Information

Assay Name:  Protease Inhibition Assay
Description:  The Ki values were determined using fluorogenic substrate, 2-(aminobenzoyl)-Thr-Ile-Nle-Phe(p-NO2)-Gln-ArgNH2. A standard curve relating changes in fluorescent intensity to changes in concentration of product was used to convert fluorescent changes into molar velocities. The amount of cleavage was determined by HPLC.
Affinity data for this assay
 

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Last update November 1, 2007
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