Assay Method Information |
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| Protease Inhibition Assay |
Description: | HIV-1 protease activity was measured by a continuous fluorometric assay using the internally quenched fluorogenic substrate DABCYL-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS. The proteolytic activity can be monitored by the increase in fluorescence intensity, and the protease products were analyzed by HPLC. |
Affinity data for this assay | |
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