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Assay Method Information

Assay Name:  Protease Inhibition Assay
Description:  HIV-1 protease activity was measured by a continuous fluorometric assay using the internally quenched fluorogenic substrate DABCYL-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS. The proteolytic activity can be monitored by the increase in fluorescence intensity, and the protease products were analyzed by HPLC.
Affinity data for this assay
 

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Last update November 1, 2007
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