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Assay Method Information

Assay Name:  Aromatase Assay
Description:  The enzyme activity was assayed by measuring the 3H-labeled H2O formed from [1,2,6,7-3H ]androstenedione / androstenedione during aromatization. After incubation, the reaction was terminated, and the charcoal-adsorbed steroids were separated by centrifugation. Aliquots of the supernatant were assayed for the presence of 3H-labeled H2O by counting using a liquid scintillation spectrometer.
Affinity data for this assay
 

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Last update November 1, 2007
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