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Assay Method Information

Assay Name:  HIV-1 RT Assay
Description:  IC50 values for wild-type and mutant RTs were obtained from a scintillation proximity assay using poly rC/biotin-dG15 and 3H-dGTP. Each value represents the mean of at least three determinations. The reproducibility of the assay was gauged using an internal standard.The IC50 of reverse transcriptase is the concentration that inhibits 50% of recombinant HIV-1 RT RNA-directed DNA polymerase activity in vitro.
Affinity data for this assay
 

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Last update November 1, 2007
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