Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Caspase Inhibition Assay |
| Description: | The substrate peptides terminating in AMC/AFC are processed by caspases with or without inhibitors, and the accumulation of AMC/AFC was assessed with a Cytofluor 4000 fluorescent plate reader (Perseptive Biosystems) at excitation and emission wavelengths of 360 and 460 nm (AMC) and 395 and 530 nm (AFC), respectively. Compounds were tested at a single dose (typically 5-50 uM), and full IC50 curves were run for all compounds expressing measurable inhibitory activity. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||