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Assay Method Information

Assay Name:  Caspase Inhibition Assay
Description:  The substrate peptides terminating in AMC are processed by caspases with or without inhibitors. The amount of AMC released was determined by using a Victor microplate fluorometer (Perkin-Elmer Life Sciences, Boston, MA) at excitation and emission wavelengths of 355 and 460 nm. Compounds were tested in duplicate, and IC50 curves were calculated for all inhibitors. Final IC50s were the average of three independent experiments.
Affinity data for this assay
 

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Last update November 1, 2007
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