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Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  The cholinesterase assays were performed using colorimetric method reported by Ellman. Enzyme activity was determined by measuring the absorbance at 420 nm after 10 min, relative to the drug-free control. Triplicate measurements were carried out at a total of 8 drug concentrations. The IC50 values were determined from a plot of percentage of inhibition vs -log [drug].
Affinity data for this assay
 

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Last update November 1, 2007
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