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Assay Method Information |
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| | CDK2/Cyclin E and CDK4/Cyclin D1 Kinase Assay |
| Description: | The enzyme was assayed with substrate GST-Rb in the presence of 10 uM ATP/[gamma-33P] ATP, and capturing the 33-P labeled reaction products on GSH-Sepharose beads. IC50 is the inhibitor concentration, which inhibits 50% of enzyme activity that catalyzes the transfer of the terminal phosphate from [gamma-33P] labeled ATP to GST-Rb. Use of 33P-labeled ATP allows this transfer to be monitored by scintillation counting. |
| Affinity data for this assay | |
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