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Assay Method Information

Assay Name:  DPP Inhibition Assay
Description:  The DPP activity resulted in the formation of the fluorescent product amidomethylcoumarin (AMC), which was monitored by excitation at 355 nm and measurement of fluorescent emission at 460 nm. For each concentration of inhibitor or DMSO control, the steady state rates were used to fit a rectangular hyperbola, from which Km and Vmax values were determined by nonlinear regression analysis. The ratio of the Km/Vmax vs inhibitor concentration was plotted and the negative X-intercept, as calculated by linear regression, was the competitive Ki.
Affinity data for this assay
 

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Last update November 1, 2007
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